Reagentes e instrumentos para imunologia, biologia celular e biologia molecular.

X-GAL [7240-90-6]

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Referência HY-15934-1g

Tamanho : 1g

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Marca : MedChemExpress

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Telefone : +1 850 650 7790

X-GAL (BCIG) is a widely used chromogenic β-galactosidase substrate. X-GAL is a colorless compound until cleaved by β-galactosidase, at which point X-GAL turns to an insoluble and detectable blue compound, making X-GAL particularly useful in techniques such as blue-white screening for cloning in bacteria. X-GAL can also be used for detection of β-galactosidase activity.

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X-GAL Chemical Structure

CAS No. : 7240-90-6

This product is a controlled substance and not for sale in your territory.

Based on 1 publication(s) in Google Scholar

Other Forms of X-GAL:

5-Bromo-4-chloro-3-indolyl-α-D-mannopyranoside, 93% Obtenir un devis

Description

In Vitro

When the cells (HUVEC for example) were grown in dishes to 80% confluence, 50 μmol/L H₂O₂ were added in the medium for 12 h.
Subsequently, cells were harvested with trypsin/EDTA and washed three times with PBS and further fixed with 4% formaldehyde for 10 min.
As control, cells without H₂O₂ treatment were prepared in the same way. Cells were stained with by X-gal at 37 ℃^[2].

The intestinal tissue was excised and cut longitudinally. After the intestinal content was rinsed off with phosphate buffer solution, the intestinal tissue was placed with the inner surface facing up on the slide.
Next, a single blue colony containing β-gal (E. coli DH5α containing pUC18) was dilution in 100 μL PBS and then 10 μL were added in the tissue.
After incubation for 1 h, the tissues were washed with PBS solution.
As control, tissues without the blue colony were prepared in the same way.
All tissues were fixed with 4% formaldehyde for 10 min and were further stained by adding X-gal at 37 ℃^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Masse moléculaire

408.63

Formule

C₁₄H₁₅BrClNO₆

CAS No.

7240-90-6

Appearance

Solid

Color

White to off-white

SMILES

O[C@H]([C@@H](O)[C@H]1O)[C@@H](O[C@@H]1CO)OC2=CNC3=C2C(Cl)=C(Br)C=C3

Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvant et solubilité

In Vitro:

DMF : 200 mg/mL (489.44 mM; Need ultrasonic)

DMSO : 100 mg/mL (244.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4472 mL	12.2360 mL	24.4720 mL
5 mM	0.4894 mL	2.4472 mL	4.8944 mL
10 mM	0.2447 mL	1.2236 mL	2.4472 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité
Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 4

Add each solvent one by one: 10% DMF 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2 mg/mL (4.89 mM); Clear solution
Protocol 5

Add each solvent one by one: 10% DMF 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2 mg/mL (4.89 mM); Clear solution
Protocol 6

Add each solvent one by one: 10% DMF 90% Corn Oil
Solubility: ≥ 2 mg/mL (4.89 mM); Clear solution

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureté et documentation

Purity: 99.88%

Select Batch:

Fiche technique (275 KB) SDS (393 KB)

COA (249 KB) HNMR (188 KB) LCMS (484 KB)

Instruction de manipulation (2659 KB)

Références

[1]. Sanchez-Ramos J, et al. The X-gal caution in neural transplantation studies. Cell Transplant. 2000 Sep-Oct;9(5):657-67. [Content Brief]

[2]. Li S, et al. In-situ SERS readout strategy to improve the reliability of beta-galactosidase activity assay based on X-gal staining in shortening incubation times. Talanta. 2021 Nov 1;234:122689. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / DMF	1 mM	2.4472 mL	12.2360 mL	24.4720 mL	61.1800 mL
	5 mM	0.4894 mL	2.4472 mL	4.8944 mL	12.2360 mL
	10 mM	0.2447 mL	1.2236 mL	2.4472 mL	6.1180 mL
	15 mM	0.1631 mL	0.8157 mL	1.6315 mL	4.0787 mL
	20 mM	0.1224 mL	0.6118 mL	1.2236 mL	3.0590 mL
	25 mM	0.0979 mL	0.4894 mL	0.9789 mL	2.4472 mL
	30 mM	0.0816 mL	0.4079 mL	0.8157 mL	2.0393 mL
	40 mM	0.0612 mL	0.3059 mL	0.6118 mL	1.5295 mL
	50 mM	0.0489 mL	0.2447 mL	0.4894 mL	1.2236 mL
	60 mM	0.0408 mL	0.2039 mL	0.4079 mL	1.0197 mL
	80 mM	0.0306 mL	0.1530 mL	0.3059 mL	0.7648 mL
	100 mM	0.0245 mL	0.1224 mL	0.2447 mL	0.6118 mL

X-GAL Related Classifications

Others

Others Metabolic Enzyme/Protease
Fluorescent Dye Glucosidase

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

X-GAL7240-90-6BCIGFluorescent DyeGlucosidaseβ-galactosidase substrateblue-white screeningInhibitorinhibitorinhibit

X-GAL [7240-90-6]

Referência HY-15934-1g

Contactar o distribuidor local :

Marca : MedChemExpress

Contactar o distribuidor local :

Other Forms of X-GAL:

X-GAL Related Antibodies

Calculateur de molarité

Calculateur de dilution

Complete Stock Solution Preparation Table

X-GAL Related Classifications

Keywords:

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