SDS precast gels with a gradient of 8-16% are important tools in the field of protein electrophoresis, designed to facilitate the separation of proteins based on their molecular weight. These gels feature a gradual increase in polyacrylamide concentration, allowing for effective resolution of a diverse range of protein sizes, particularly those that fall within the medium molecular weight category.

Key Features

1. Gradient Structure: The 8-16% gradient provides an optimal environment for separating proteins ranging from approximately 10 kDa to 100 kDa. The larger pores at the top of the gel accommodate larger proteins, while the smaller pores at the bottom help resolve smaller proteins, ensuring clear separation.
2. Precast Convenience: These gels come ready-to-use, eliminating the time and variability associated with hand-casting. This feature enhances reproducibility and streamlines laboratory workflows, making them ideal for high-throughput applications.
3. Versatile Applications: SDS precast gels with an 8-16% gradient are suitable for various applications, including SDS-PAGE for denaturing protein analysis and Western blotting for protein detection. They are particularly useful for analyzing complex samples containing a mixture of proteins.
4. Enhanced Performance: These gels are designed to provide high-resolution separation, yielding sharp and distinct bands that are crucial for accurate analysis in subsequent techniques. Their performance is often superior to traditional single-percentage gels, especially when dealing with samples of unknown or varying sizes.