Antifade Mounting Medium with DAPI

Referência CRG1030-10ml

Tamanho : 10ml

Marca : Cohesion Biosciences

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Product NameAntifade Mounting Medium with DAPI
Cat No: CRG1030
Source:
Reactivity:
Applications: IF
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Description: Antifade Mounting Medium with DAPI is an aqueous anti-fade mounting medium designed to preserve fluorescence when imaging tissues and cell samples. It is fortified with DAPI as a counter-stain for DNA (DAPI also stains RNA).
Form: Liquid
Directions for Use : 1. Bring the vial to room temperature. 2. Rinse slide to be mounted with distilled or deionized water, touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface. 3. Apply 3-4 drops of mounting medium directly on top of the specimen and spread out evenly by tilting slide back and forth or spread evenly with a 0.2 ml plastic pipette tip making sure the tissue is not touched. Excess medium can be removed by touching the edges of slide against paper towel. 4. Let stand at room temperature for about 5 minutes. 5. Apply cover slip carefully avoiding air bubbles. 6. The specimen is ready for visualization under a microscope.
Storage/Stability : Shipped at 4°C. Store at -20°C for one year. Protect from light. Avoid freeze/thaw cycles.