Sample Type:|Serum||Intended Use:|This kit can be used for quantitative determination of ethanol in human serum.||Range: |0-100mg/dL, 0-21.5mmol/L，r2>0.990||Test Principle:|On the condition of ethanol dehydrogenase, ethanol will react with NAD+ to produce ethanol and NADH, and the NADH will change the absorbance at 340nm wavelength. The concentration of ethanol in serum (plasma) can measured by detecting the change of the absorbance at 340nm wavelength.||Accuracy: |Relative deviation ≤15.0%||Precision: |Intra-Assay CV: <8%|Inter-Assay CV: <10%||Kit Components (see Protocol for final kit quantities):|Reagent 1, Powder:|NAD, ≥5mmol/L|Ethanol dehydrogenase, ≥500U/L||Reagent 2, Buffer:|Tris-HCl buffer, pH9.0||Reagent 3, Standard:|Ethanol, 95%||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.