Bst II DNA Polymerase
Referência LP301-01
Tamanho : 800units×1
Marca : TransGen Biotech
This product contains Bst II DNA polymerase, 5×LAMP Reaction Mix, fluorescent dye TS LAMP Green. It is suitable for LAMP reaction with DNA as template, featuring strong amplification ability and high specificity, and has excellent reaction performance in LAMP reactionusing fluorescence quantitative method (dye method, probe method).
Bst II DNA Polymerase is a recombinant Bacillus stearothermophilus DNA polymerase, which is obtained by purification and isolation after expression in E. coli. The enzyme has 5'→3' DNA polymerase activity and lacks 5'→3' exonucleaseactivity.
5×LAMP Reaction Mix is an optimized reaction mastermix, which already contains MgSO4 , dNTPs and other components required for the reaction, saving the time of addition.
TS LAMP Green is a DNA-binding dye for fluorescence quantitative amplification. It has a similar spectrumto SYBR Green I and is compatible with all well-known brands of qPCR instruments. Replacing SYBR Green I with TS LAMP Green does not require any changes to your current procedures and equipment.
Highlights
· Loop-mediated Isothermal Amplification, LAMP
· Fast polymerization
· Strongstrand-displacement capability
Application
· DNA isothermal amplification
· DNA sequencing through high GC regions
· Rapid sequencing from nanogram amounts of DNA template
· Applicablefor experiments requiring mesophilic strand-displacement
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)
Component | LP301-01-V2 (100 rxns) | LP301-02-V2 (200 rxns) |
Bst II DNA Polymerase | 200 μl | 400 μl |
5×LAMP Reaction Mix | 0.6 ml | 1.2 ml |
TS LAMP Green (20×) | 100 μl | 200 μl |
6×DNA Loading Buffer | 500 μl | 1 ml |
Nuclease-free Water | 2×1 ml | 4×1 ml |