Reagentes e instrumentos para imunologia, biologia celular e biologia molecular.

CD62p (LECAM, P-Selectin, Endothelial Leukocyte Adhesion Molecule) (PE)

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Referência C2415-55P-PE-100ul

Tamanho : 100ul

Contactar o distribuidor local :

Telefone : +1 850 650 7790

Marca : US Biological

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Contactar o distribuidor local :

Telefone : +1 850 650 7790

C2415-55P-PE CD62p (LECAM, P-Selectin, Endothelial Leukocyte Adhesion Molecule) (PE)

Clone Type

Polyclonal

Host

mouse

Source

human

Swiss Prot

P16109

Isotype

IgG1

Grade

Affinity Purified

Applications

FC IP

Crossreactivity

Hu Po Sh

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

CD62P is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leukocytes and endothelial cells.||Applications:|Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications have not been tested. ||Recommended Dilutions: |Optimal dilution to be determined by researcher.||Hybridoma:|Spleen cells from immunized CD62P knock-out mice (strain C57/B6) were fused with cells of the NS-1 myeloma cell line.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications

Product Type: Mab|Isotype: IgG1|Clone No: 9A151 (Psel.KO.2.5)|Host: mouse|Source: human|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).|Purity: Purified by Protein A affinity chromatography from tissue culture supernatant.|Immunogen: CD62P transfected 300.19 cells.|Specificity: Recognizes the human CD62P cell surface antigen, a 140kD glycoprotein also known as P-selectin. Species Crossreactivity: porcine, sheep||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

CD62P transfected 300.19 cells.

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).

Purity

Purified by Protein A affinity chromatography from tissue culture supernatant.

Specificity

Recognizes the human CD62P cell surface antigen, a 140kD glycoprotein also known as P-selectin. Species Crossreactivity: porcine, sheep

References

1. Massaguer, A. et al. (2000) Tissue antigens 56:117-128. 2. Massaguer, A. et al. (2003) Immunopathol. 96:169-181. 3.Krajewski, S. et al. (2012) Flow cytometry analysis of porcine platelets: optimized methods for best results. Platelets. 23: 386-94. 4. Johnson, C.A. Jr et al. (2011) Platelet activation in ovines undergoing sham surgery or implant of the second generation PediaFlow pediatric ventricular assist device. Artif Organs. 35 (6): 602-13. 5.Tunjungputri, R.N. et al. (2016) Invasive pneumococcal disease leads to activation and hyperreactivity of platelets. Thromb Res. 144: 123-6. 6.Shankarraman, V. et al. (2014) Biocompatibility Assessment of the CentriMag-Novalung Adult ECMO Circuit in a Model of Acute Pulmonary Hypertension. ASAIO J. 60 (4): 429-35. 7.Chan, C.H.H. et al. (2017) Shear Stress-Induced Total Blood Trauma in Multiple Species. Artif Organs. 41 (10): 934-47. 8.Johnson, C.A. Jr. et al. (2011) Platelet activation in ovines undergoing sham surgery or implant of the second generation PediaFlow pediatric ventricular assist device. Artif Organs. 35 (6): 602-13.