Cxcl1 ELISA Kit (Rat)

Referência OKEH02715

Tamanho : 96Wells

Marca : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Cxcl1 ELISA Kit (Rat) (OKEH02715) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cxcl1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Cxcl1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cxcl1 captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.1% (n = 20)
Mean Inter-assay CV%: < 8.3% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Cxcl1 Microplate96 Wells (12 x 8 Well strips)
Cxcl1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Cxcl1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity7.96 pg/mL
SpecificityNatural and recombinant Rat Growth-regulated alpha protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCxcl1
Gene Full NameC-X-C motif chemokine ligand 1
Alias Symbolschemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha);CINC-1;C-X-C motif chemokine 1;cytokine-induced neutrophil chemoattractant 1;gro;Gro1;growth-regulated alpha protein;platelet-derived growth factor-inducible protein KC.
NCBI Gene Id81503
Protein NameP14095
Description of TargetGrowth-regulated alpha protein
Uniprot IDhttps://www.uniprot.org/uniprot/P14095
Protein Accession #NP_110472.1
Nucleotide Accession #NM_030845.1
Protein Size (# AA)96
Molecular Weight10 kDa

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Price Bef. VAT
OKBB00164
 96wells/kit,withremovablestrips. 
OKBB00365
 96wells/kit,withremovablestrips.