ER-Tracker Green [730931-46-1]
Referência HY-D1297-5mg
Tamanho : 5mg
Marca : MedChemExpress
ER-Tracker dye is a derivative of BODIPY series dyes coupled with Glibenclamide (HY-15206), highly selective binding to the endoplasmic reticulum, non-toxic to cells at low concentrations, this type of dye is an environmentally sensitive probe, and formaldehyde treatment can still retain part of the fluorescence, with high fluorescence life, good extinction coefficient and other characteristics. Glibenclamide is an atp-dependent K+ channel blocker (Kir6, KATP) and CFTR Cl-channel blocker that binds in the endoplasmic reticulum. ER-Tracker is not suitable for staining cells after fixation.
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ER-Tracker Green Chemical Structure
CAS No. : 730931-46-1
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Based on 1 publication(s) in Google Scholar
Description |
ER-Tracker dye is a derivative of BODIPY series dyes coupled with Glibenclamide (HY-15206), highly selective binding to the endoplasmic reticulum, non-toxic to cells at low concentrations, this type of dye is an environmentally sensitive probe, and formaldehyde treatment can still retain part of the fluorescence, with high fluorescence life, good extinction coefficient and other characteristics. Glibenclamide is an atp-dependent K+ channel blocker (Kir6, KATP) and CFTR Cl-channel blocker that binds in the endoplasmic reticulum. ER-Tracker is not suitable for staining cells after fixation[1]. |
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In Vitro |
1. Preparation of ER-Tracker working solution MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Masse moléculaire |
783.09 |
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Formule |
C37H42BClF2N6O6S |
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CAS No. |
730931-46-1 |
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Appearance |
Solid |
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Color |
Brown to reddish brown |
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Emission (Em) |
520 |
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Excitation (Ex) |
489 |
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SMILES |
O=C(NS(=O)(C1=CC=C(CCNC(C2=C(OC)C(NC(CCC3=CC=C4C=C5C(C)=CC(C)=35[B+3]([F-])([N-]43)[F-])=O)=CC(Cl)=C2)=O)C=C1)=O)NC6CCCCC6 |
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Livraison | Room temperature in continental US; may vary elsewhere. |
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Stockage |
4°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |
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Solvant et solubilité |
In Vitro:
DMSO : 50 mg/mL (63.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration:
mg/mL
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Pureté et documentation | |||||||||||||
Références |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO | 1 mM | 1.2770 mL | 6.3850 mL | 12.7699 mL | 31.9248 mL |
5 mM | 0.2554 mL | 1.2770 mL | 2.5540 mL | 6.3850 mL | |
10 mM | 0.1277 mL | 0.6385 mL | 1.2770 mL | 3.1925 mL | |
15 mM | 0.0851 mL | 0.4257 mL | 0.8513 mL | 2.1283 mL | |
20 mM | 0.0638 mL | 0.3192 mL | 0.6385 mL | 1.5962 mL | |
25 mM | 0.0511 mL | 0.2554 mL | 0.5108 mL | 1.2770 mL | |
30 mM | 0.0426 mL | 0.2128 mL | 0.4257 mL | 1.0642 mL | |
40 mM | 0.0319 mL | 0.1596 mL | 0.3192 mL | 0.7981 mL | |
50 mM | 0.0255 mL | 0.1277 mL | 0.2554 mL | 0.6385 mL | |
60 mM | 0.0213 mL | 0.1064 mL | 0.2128 mL | 0.5321 mL |
ER-Tracker Green Related Classifications
- Others
- Fluorescent Dye
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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
ER-Tracker Green730931-46-1Fluorescent DyefluorescentdyeendoplasmicreticulumInhibitorinhibitorinhibit