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> FLAG peptide [98849-88-8]

FLAG peptide [98849-88-8]

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Referência HY-P0223-10mg

Tamanho : 10mg

Marca : MedChemExpress

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FLAG peptide is a multifunctional fusion tag for the purification of recombinant proteins. FLAG peptide maintances the natural folding of its fusing proteins. FLAG peptide can be removed by enterokinase, and eluted under non-denaturing conditions.

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Synthèse de peptides personnalisée

FLAG peptide Chemical Structure

FLAG peptide Chemical Structure

CAS No. : 98849-88-8

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Based on 13 publication(s) in Google Scholar

Other Forms of FLAG peptide:

  • FLAG peptide TFA Obtenir un devis
Description

FLAG peptide is a multifunctional fusion tag for the purification of recombinant proteins. FLAG peptide maintances the natural folding of its fusing proteins. FLAG peptide can be removed by enterokinase, and eluted under non-denaturing conditions[1].

In Vitro

Flag peptide is a highly acidic octapeptide that has a calcium-dependent epitope for monoclonal antibodies, allows elution under non-denaturing conditions, and can be fused to the N-terminus of the target protein. Flag peptide can be captured by the immunoaffinity column in the presence of Ca2+ and eluted by EDTA at low concentrations and neutral pH (thus close to physiological conditions)[1][2].
Reprogramming factors in mammalian cells are purified by FLAG peptide[3] (1) Transfected cells: HEK293 cells were transfected with FLAG-tagged reprogramming factors, and washed with PBS twice 24 h after transfection. (2) Collect cells: With NP40 cell lysis buffer (50 mM Tris-HCl, pH 7.4, 250 mM NaCl, 5 mM EDTA, 1% NP-40, and protease inhibitors). Centrifuge the cell lysate at 14,500 rpm for 15 min at 4 °C to collect the soluble fraction. (3) Add Anti-Flag Affinity Gel(HY-K0217) to the supernatant and incubate in a cold room with rotation for 16 h. (4) Wash twice with PBS and add 0.1 mg/ml FLAG peptide to elute and retain the FLAG-tagged protein. (5) The eluted protein is dialysed with a dialysis tubing in dialysis buffer (PBS).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

FLAG peptide Related Antibodies
Masse moléculaire

1012.97

Formule

C41H60N10O20
CAS No.

98849-88-8
Appearance

Solid

Color

White to off-white

Sequence

Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys
Sequence Shortening

DYKDDDDK
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Sealed storage, away from moisture and light, under nitrogen

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

Solvant et solubilité
In Vitro: 

H2O : 100 mg/mL (98.72 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.9872 mL 4.9360 mL 9.8720 mL
5 mM 0.1974 mL 0.9872 mL 1.9744 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Calculateur de molarité

  • Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (98.72 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Pureté et documentation

Purity: 99.74%

COA (265 KB) RP-HPLC (243 KB) Elemental Analysis Report (124 KB)

Instruction de manipulation (2659 KB)
Références

  • [1]. Einhauer A, et al. The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins. J Biochem Biophys Methods. 2001 Oct 30;49(1-3):455-65.  [Content Brief]

    [2]. Schuster M, et al. Protein expression in yeast; comparison of two expression strategies regarding proteinmaturation. J Biotechnol. 2000 Dec 28;84(3):237-48.  [Content Brief]

    [3]. Min-Joon Han, et al. Purification of functional reprogramming factors in mammalian cell using FLAG –Tag. Biochem Biophys Res Commun. 2017 Oct 14;492(2):154-160.  [Content Brief]

  • [1]. Einhauer A, et al. The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins. J Biochem Biophys Methods. 2001 Oct 30;49(1-3):455-65.

    [2]. Schuster M, et al. Protein expression in yeast; comparison of two expression strategies regarding proteinmaturation. J Biotechnol. 2000 Dec 28;84(3):237-48.

    [3]. Min-Joon Han, et al. Purification of functional reprogramming factors in mammalian cell using FLAG –Tag. Biochem Biophys Res Commun. 2017 Oct 14;492(2):154-160.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.9872 mL 4.9360 mL 9.8720 mL 24.6799 mL
5 mM 0.1974 mL 0.9872 mL 1.9744 mL 4.9360 mL
10 mM 0.0987 mL 0.4936 mL 0.9872 mL 2.4680 mL
15 mM 0.0658 mL 0.3291 mL 0.6581 mL 1.6453 mL
20 mM 0.0494 mL 0.2468 mL 0.4936 mL 1.2340 mL
25 mM 0.0395 mL 0.1974 mL 0.3949 mL 0.9872 mL
30 mM 0.0329 mL 0.1645 mL 0.3291 mL 0.8227 mL
40 mM 0.0247 mL 0.1234 mL 0.2468 mL 0.6170 mL
50 mM 0.0197 mL 0.0987 mL 0.1974 mL 0.4936 mL
60 mM 0.0165 mL 0.0823 mL 0.1645 mL 0.4113 mL
80 mM 0.0123 mL 0.0617 mL 0.1234 mL 0.3085 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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FLAG peptide Related Classifications

  • Inflammation/Immunology
Help & FAQs

Keywords:

FLAG peptide98849-88-8OthersInhibitorinhibitorinhibit

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