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> G-418 (disulfate) [108321-42-2]

G-418 (disulfate) [108321-42-2]

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G-418 disulfate (Geneticin sulfate), is an aminoglycoside antibiotic, inhibits protein synthesis in eukaryotes and prokaryotes. G-418 disulfate is commonly used as a selective agent for eukaryotic cells.

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G-418 disulfate Chemical Structure

G-418 disulfate Chemical Structure

CAS No. : 108321-42-2

This product is a controlled substance and not for sale in your territory.

Based on 34 publication(s) in Google Scholar

Other Forms of G-418 disulfate:

  • G-418 Obtenir un devis

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Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam
Description

G-418 disulfate (Geneticin sulfate), is an aminoglycoside antibiotic, inhibits protein synthesis in eukaryotes and prokaryotes. G-418 disulfate is commonly used as a selective agent for eukaryotic cells[1].

IC50 & Target

Aminoglycoside

 

In Vitro

G418 sulfate, an aminoglycoside neomycin analogue, interferes with protein synthesis and has been used extensively for selection of mammalian cell lines that possess neomycin resistance (NR). The neomycin resistance (neo) gene is frequently used in eukaryotic vectors as a dominant selectable gene. G418 can be covalently bound to asialoorosomucoid (AsOR) to form a conjugate for hepatocyte-specific targeting and toxicity[2].
The human GD3 synthase cDNA was transfected into MDA-MB231 cells, and G-418 bulk selection was used to select cells stably overexpressing the GD3 synthase[2]. An aminoglycoside antibiotic, G418, has been shown to be an inhibitor of many pro- and eukaryotes at concentrations from 1-300 microgram/ml[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

G-418 disulfate Related Antibodies
Masse moléculaire

692.71

Formule

C20H44N4O18S2
CAS No.

108321-42-2
Appearance

Solid

Color

White to off-white

SMILES

O=S(O)(O)=O.O[C@@H]([C@@H]1O[C@@]([C@@H]([C@@H](O)[C@@H]2O)N)(07)O[C@]2(07)[C@H](O)C)[C@H]([C@@H](C[C@@H]1N)N)O[C@](OC[C@](C)(O)[C@@H]3NC)(07)[C@@H]3O.O=S(O)(O)=O
Structure Classification
  • Antibiotics
  • Disease Research
  • Antibacterial
  • Antibiotics
  • Disease Research
  • Antifungal
  • Antibiotics
  • Other Antibiotics
  • Antibiotics
  • Plant Cell Culture
  • Antibiotics
  • Resistance Selection
Initial Source
  • Microorganisms

Micromonospora rhodorangea
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, stored under nitrogen, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)

Solvant et solubilité
In Vitro: 

H2O : 125 mg/mL (180.45 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4436 mL 7.2180 mL 14.4361 mL
5 mM 0.2887 mL 1.4436 mL 2.8872 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Calculateur de molarité

  • Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (72.18 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Pureté et documentation

Purity: 99.75%

SDS (393 KB)

COA (265 KB) HNMR (288 KB) RP-HPLC (278 KB)

Instruction de manipulation (2659 KB)
Références

  • [1]. Giordano-Santini R, et al. An antibiotic selection marker for nematode transgenesis. Nat Methods. 2010;7(9):721-723.  [Content Brief]

    [2]. Volarevic M, et al. A novel G418 conjugate results in targeted selection of genetically protected hepatocytes without bystander toxicity. Bioconjug Chem. 2007;18(6):1965-1971.  [Content Brief]

    [3]. Kwon KM, et al. Disialyl GD2 ganglioside suppresses ICAM-1-mediated invasiveness in human breast cancer MDA-MB231 cells. Int J Biol Sci. 2017;13(3):265-275. Published 2017 Feb 12.  [Content Brief]

    [4]. Davies J, et al. A new selective agent for eukaryotic cloning vectors. Am J Trop Med Hyg. 1980;29(5 Suppl):1089-1092.  [Content Brief]

Administration animale
[3]

To characterize the sensitivity of the trypanosome populations to G418 in vivo, bloodstream forms of T. brucei brucei GUTat 3.1 and T. brucei brucei GUTat 3.1/BBR3 are expanded separately in sublethally irradiated mice. Prior to the first peak of parasitemia, trypanosomes are collected, and aliquots containing 106 trypanosomes are inoculated intraperitoneally into mice. Twenty-four hours following infection, the mice are divided into groups and treated with G418 at a dose of 10, 20, 30, 40, 50, or 80 mg/kg of body weight (bw) by inoculating intraperitoneally 0.2 mL of the drug in sterile water. At 24 and 48 h following the first treatment, G418 is administered to animals in each group at the same dose as before, resulting in three treatments per mouse. Repeated drug treatments are necessary to ensure complete elimination of nontransfected GUTat 3.1 parasites from the mice. Mice are then monitored daily, for 33 days, for the presence of parasites by microscopic examination of wet-blood films. Animals found to be parasitemic are recorded and then removed from the experiment.

MCE n'a pas confirmé de manière indépendante l'exactitude de ces méthodes. Ils sont pour référence seulement.
Références

  • [1]. Giordano-Santini R, et al. An antibiotic selection marker for nematode transgenesis. Nat Methods. 2010;7(9):721-723.  [Content Brief]

    [2]. Volarevic M, et al. A novel G418 conjugate results in targeted selection of genetically protected hepatocytes without bystander toxicity. Bioconjug Chem. 2007;18(6):1965-1971.  [Content Brief]

    [3]. Kwon KM, et al. Disialyl GD2 ganglioside suppresses ICAM-1-mediated invasiveness in human breast cancer MDA-MB231 cells. Int J Biol Sci. 2017;13(3):265-275. Published 2017 Feb 12.  [Content Brief]

    [4]. Davies J, et al. A new selective agent for eukaryotic cloning vectors. Am J Trop Med Hyg. 1980;29(5 Suppl):1089-1092.  [Content Brief]

  • [1]. Giordano-Santini R, et al. An antibiotic selection marker for nematode transgenesis. Nat Methods. 2010;7(9):721-723.

    [2]. Volarevic M, et al. A novel G418 conjugate results in targeted selection of genetically protected hepatocytes without bystander toxicity. Bioconjug Chem. 2007;18(6):1965-1971.

    [3]. Kwon KM, et al. Disialyl GD2 ganglioside suppresses ICAM-1-mediated invasiveness in human breast cancer MDA-MB231 cells. Int J Biol Sci. 2017;13(3):265-275. Published 2017 Feb 12.

    [4]. Davies J, et al. A new selective agent for eukaryotic cloning vectors. Am J Trop Med Hyg. 1980;29(5 Suppl):1089-1092.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.4436 mL 7.2180 mL 14.4361 mL 36.0901 mL
5 mM 0.2887 mL 1.4436 mL 2.8872 mL 7.2180 mL
10 mM 0.1444 mL 0.7218 mL 1.4436 mL 3.6090 mL
15 mM 0.0962 mL 0.4812 mL 0.9624 mL 2.4060 mL
20 mM 0.0722 mL 0.3609 mL 0.7218 mL 1.8045 mL
25 mM 0.0577 mL 0.2887 mL 0.5774 mL 1.4436 mL
30 mM 0.0481 mL 0.2406 mL 0.4812 mL 1.2030 mL
40 mM 0.0361 mL 0.1805 mL 0.3609 mL 0.9023 mL
50 mM 0.0289 mL 0.1444 mL 0.2887 mL 0.7218 mL
60 mM 0.0241 mL 0.1203 mL 0.2406 mL 0.6015 mL
80 mM 0.0180 mL 0.0902 mL 0.1805 mL 0.4511 mL
100 mM 0.0144 mL 0.0722 mL 0.1444 mL 0.3609 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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G-418 disulfate Related Classifications

  • Infection
  • Anti-infection
  • Bacterial Antibiotic
Help & FAQs

Keywords:

G-418 disulfate108321-42-2GeneticinAntibiotic G-418Antibiotic G418Antibiotic G 418BacterialAntibioticproteinsynthesiseukaryoticcellsInhibitorinhibitorinhibit

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