Glyceraldehyde 3 Phosphate Dehydrogenase (GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685) (HRP)

Referência G8140-01G-HRP-100ul

Tamanho : 100ul

Marca : US Biological

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G8140-01G-HRP Glyceraldehyde 3 Phosphate Dehydrogenase (GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685) (HRP)

Clone Type
Polyclonal
Host
mouse
Swiss Prot
P04406
Isotype
IgG1
Grade
Affinity Purified
Applications
E
Crossreactivity
Bo Ca Fe Gt Hu Mo Po Rb Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kD subunits.||Applications: |Suitable for use in ELISA. Other applications not tested.||Recommended Dilution:|Optimal dilutions to be determined by the researcher.||Matched Pairs:|Capture: G8140-01H, G8140-01I|Detection: G8140-01G||Hybridoma: |Sp2/0 myeloma cells with spleen cells from Balb/c mice.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. |For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 9L274|Host: mouse|Concentration: As reported|Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with horseradish peroxidase (HRP).|Purity: Purified by Protein A affinity chromatography.|Immunogen: Rabbit muscle GAPDH.|Specificity: Recognizes GAPDH monomer (36kD) and the dimer form. Does not react with the tetrameric form. Species crossreactivity: human, bovine, pig, goat, dog, rabbit, cat, rat, mouse, and fish.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Rabbit muscle GAPDH.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes GAPDH monomer (36kD) and the dimer form. Does not react with the tetrameric form. Species crossreactivity: human, bovine, pig, goat, dog, rabbit, cat, rat, mouse, and fish.
References
FEBS Letters, (1992), Volume 300, number 1, 9-12.