Human IFN-gamma ELISA Kit (96-well)

Referência EA100042

Tamanho : 1x96wells

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Human IFN-gamma ELISA Kit (96-well)

Specifications
Product Data
ACCN NM_000619
Assay Length 3 hours
Assay Platform 96-well plate
Assay Type Solid Phase Sandwich ELISA
Assay Type Sandwich
Background

Interferon gamma (IFN-.) is a multifunctional protein first observed as an antiviral activity in cultures of Sindbis virus-infected human leukocytes stimulated by PHA . Produced by Tlymphocytes and natural killer (NK) cells, IFN-. .is now known to be both an inhibitor of viral replication and a regulator of numerous immunological functions. Human IFN-.. is reported to be active only on human and non-human primate cells . The biochemistry and biological activities of the interferons have been extensively reviewed .

Human IFN-. .is a 143 amino acid residue, 20 or 25 kDa glycoprotein that demonstrates little sequence homology to IFN-.. or -... Naturally occurring IFN-. .is found as either of two molecular weight species, differing in degree of glycosylation. Human IFN-.. apparently exists as a head-to-tail dimer in solution with the C-terminus of one monomer aligned with the N-terminus of the other monomer A receptor for IFN-. has been identified and its gene localized to chromosome 6 Apparently the product of a single gene, the receptor is a single chain 90 kDa glycoprotein that shows a high degree of species-specific binding of IFN-...

Functionally, IFN-. .produces a variety of effects. Produced by CD8+, NK, gd, and TH1 T helper cells, IFN-. .has documented antiviral, antiprotozoal and immunomodulatory effects on cell proliferation and apoptosis, as well as the stimulation and repression of a variety of genes he antiprotozoal activity of IFN-. .against Toxoplasma and Chlamydia is believed to result from indoleamine 2,3-dioxygenase activity, an enzyme induced by IFN-. ..The immunomodulatory effects of IFN-.. are extensive and diverse. In monocyte/macrophages, the activities of IFN-. .include: increasing the expression of class I and II MHC antigens; increasing the production of IL-1, platelet-activating factor, H2O2, and pterin; protection of monocytes against LAK cell-mediated lysis; downregulation of IL-8 mRNA expression that is upregulated by IL-2; and, with lipopolysaccharide, induction of NO production.Finally, IFN-..has been shown to upregulate ICAM-1, but not E-Selectin or VCAM-1, expression on endothelial cells.

Components
  • Aluminium pouches with a Microwell Plate coated with antibody to human IFN-? (8 x 12)
  • 2 vials human IFN-? Standard lyophilized, 4000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-human IFN-? antibody
  • 2 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 4 pieces Adhesive Films
  • package insert
Curve Range 15.625-1000pg/ml
figure_legend Representative standard curve for IFN-? ELISA. IFN-? was diluted in serial two-fold steps in Sample Diluent.
Format 96-well strip plate
Gene Symbol IFNG
Interference No significant interference observed with available related molecules.
Locus ID 3458
Reactivity Human
Reactivity Human
Reactivity Human
Sample Type Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume 20 uL
Sensitivity 7pg/mL
Signal Colorimetric
Species Human
Specificity Natural and recombinant Human IFN-? Ligand
Standard Curve /assets/images/elisa/kits/128/che0017-096-1-e.jpg
Target Symbol IFNG
Description Human IFN-gamma ELISA Kit (96-well)
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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