IgA, alpha (AP)

Referência I1890-65E-1mg

Tamanho : 1mg

Marca : US Biological

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I1890-65E Rabbit Anti-IgA, alpha (AP)|

Clone Type
Polyclonal
Host
goat
Source
rat
Isotype
IgG
Grade
Affinity Purified
Applications
DB E IHC WB
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Applications: |Suitable for use in Immunoblot (Western or Dot Blot), ELISA and Immunohistochemistry. Other applications not tested. ||Recommended Dilutions: |ELISA: 1:2000 to 1:8000. 1.0 ug of rat IgA was used in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at RT. |Optimal dilution determined by the researcher. ||Storage and Stability:|Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Applications
Product Type: Pab|Isotype: IgG|Host: goat|Source: rat|Concentration: ~1mg/ml|Form: Supplied as a liquid in 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 50% glycerol. Label: Alkaline Phosphatase (Calf Intestine) (MW 140kD)|Purity: Purified by immunoaffinity chromatography.|Immunogen: Rat IgA a chain|Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rat IgA and Rat Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other Rat or human heavy or light chain isotypes.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Rat IgA a chain
Form
Supplied as a liquid in 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 50% glycerol. Label: Alkaline Phosphatase (Calf Intestine) (MW 140kD)
Purity
Purified by immunoaffinity chromatography.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rat IgA and Rat Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other Rat or human heavy or light chain isotypes.
References
Avarameas and Ternyrock, Immunochemistry 32; 1175 1971.