Insulin ELISA Kit (Monkey)
Referência OKCA00217
Tamanho : 96Wells
Marca : Aviva Systems Biology
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Telefone : +1 850 650 7790
| Predicted Species Reactivity | Monkey | ||||||||||||||||||||||||||||||||||||||||||
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| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Duration | 2.5 Hours | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Insulin ELISA Kit (Monkey) (OKCA00217) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A Monkey monoclonal antibody specific for Monkey Insulin has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells and incubated. After washing, a biotinylated detector antibody specific for Monkey Insulin is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Monkey Insulin captured in well. | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Range | 8 uIU/mL - 140 uIU/mL | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Reproducibility |
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| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||||||||||||||||||||||
| Sample Type | Serum, Plasma | ||||||||||||||||||||||||||||||||||||||||||
| Sensitivity | 5 uIU/mL | ||||||||||||||||||||||||||||||||||||||||||
| Specificity | This assay has high sensitivity and excellent specificity for detection of monkey INS. No significant cross-reactivity or interference between monkey INS and analogues was observed. | ||||||||||||||||||||||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay | ||||||||||||||||||||||||||||||||||||||||||
| Gene Symbol | Insulin |
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