KHOS-NP
Marca : CLS Cell Lines Service
KHOS-NP Cells
General information
Description | This cell line was derived from the HOS cell line (TE-85) by transformation using Kirsten murine sarcoma virus (Ki-MSV). The cells exhibit a high saturation density, a high plating efficiency in soft agar and produce tumors in nude mice. The cells are useful producing MSV pseudotypes with various ecotropic and xenotropic murine leukemia viruses. Cells carry the Ki-MSV genome but do not produce infectious virus particles or viral antigens. |
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Organism | Human |
Tissue | Bone |
Disease | Osteosarcoma |
Synonyms | KHOS/NP, KHOS NP, KHOSNP, R-970-5, KHOS |
Characteristics
Age | 13 years |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Fibroblast-like |
Growth properties | Monolayer, adherent |
Identifiers / Biosafety / Citation
Citation | KHOS-NP (Cytion catalog number 300235) |
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Biosafety level | 1 |
Expression / Mutation
Tumorigenic | Yes, in nude mice. |
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Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:4 is recommended |
Seeding density | 2 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | Amelogenin: x,x CSF1PO: 12 D13S317: 12 D16S539: 10,13 D5S818: 13 D7S820: 11,12 TH01: 6 TPOX: 8,11 vWA: 18 D3S1358: 15 D21S11: 31.2,32.2 D18S51: 17 Penta E: 7,12 Penta D: 9,10 D8S1179: 11,14 FGA: 24 |