In 1959, McCoy and his coworkers reported the amino acid requirements for in vitro cultivation of Novikoff Hepatoma Cells. These studies were performed using Basal Medium 5A, and subsequently modified to create a new medium known as McCoy’s 5A Medium. This media has been employed to support the growth of primary cultures derived from adrenal glands, bone marrow (normal), gingiva, lung, mouse kidney, omentum, skin, spleen and other tissues. It is a general purpose medium for primary and established cell lines.||Appearance: |Pale Yellow to orange, homogeneous, free flowing powder.||Solubility: |Pale orange, clear, complete||pH: |4.8-5.5||Endotoxin: |≤1EU/ml