Mouse Anti-Human CD57-AF647

Referência 9665-31

Tamanho : 100tests

Marca : Southern Biotech

Contactar o distribuidor local :


Telefone : +1 850 650 7790

More Information
Clone NK-1
Isotype Mouse IgMκ
Isotype Control 0101-31
Specificity Human CD57
Alternative Names HNK-1, Leu-7, B3GAT1
Description CD57 represents a 110 kDa oligosaccharide antigenic determinant present on a variety of polypeptides, lipids, and chondroitan sulfate proteoglycans. It is expressed on 7-35% of normal peripheral blood lymphocytes, including subsets of NK cells and CD8+ T lymphocytes. The carbohydrate is expressed on various cell adhesion molecules in the nervous system and is suggested to play a role in cell–cell and cell–substrate interactions.
Immunogen Membrane extract of the human lymphoblastoid cell line HSB-2
Conjugate AF647 (Alexa Fluor® 647)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Flow Cytometry – Quality tested 1-4
Immunohistochemistry-Frozen Sections – Reported in literature 5
Immunohistochemistry-Paraffin Sections – Reported in literature 6-8
Immunocytochemistry – Reported in literature 2,3
Immunoprecipitation – Reported in literature 8
Western Blot – Reported in literature 2,3,8,9
Purification – Reported in literature 9
Blocking – Reported in literature 2,8
Adhesion – Reported in literature 2

RRID Number AB_2797059
Gene ID 27087 (Human)
Gene ID Symbol B3GAT1 (Human)
Gene ID Aliases CD57; GLCATP; GLCUATP; HNK1; LEU7; NK-1; NK1
UniProt ID Q9P2W7 (Human
UniProt Name B3GA1_HUMAN (Human)
Documentation
Technical Bulletin Safety Datasheet

Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view

  1. 1. Abo T, Balch CM. A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1). J Immunol. 1981;127:1024-9. (Immunogen, FC)
  2. 2. Miura R, Ethell IM, Yamaguchi Y. Carbohydrate-protein interactions between HNK-1-reactive sulfoglucuronyl glycolipids and the proteoglycan lectin domain mediate neuronal cell adhesion and neurite outgrowth. J Neurochem. 2001;76:413-24. (FC, ICC, WB, Block, Adhesion)
  3. 3. Yanagisawa M, Taga T, Nakamura K, Ariga T, Yu RK. Characterization of glycoconjugate antigens in mouse embryonic neural precursor cells. J Neurochem. 2005;95:1311-20. (FC, ICC, WB)
  4. 4. Bentebibel S, Schmitt N, Banchereau J, Ueno H. Human tonsil B-cell lymphoma 6 (BCL6)-expressing CD4+ T-cell subset specialized for B-cell help outside germinal centers. Proc Natl Acad Sci USA. 2011;108:E488-97. (FC)
  5. 5. Liu AY, Roudier MP, True LD. Heterogeneity in primary and metastatic prostate cancer as defined by cell surface CD profile. Am J Pathol. 2004;165:1543-56. (IHC-FS)
  6. 6. Jensen HK, Donskov F, Marcussen N, Nordsmark M, Lundbeck F, von der Maase H. Presence of intratumoral neutrophils is an independent prognostic factor in localized renal cell carcinoma. J Clin Oncol. 2009;27:4709-17. (IHC-PS)
  7. 7. Zou Y, Chen T, Han M, Wang H, Yan W, Song G, et al. Increased killing of liver NK cells by Fas/Fas ligand and NKG2D/NKG2D ligand contributes to hepatocyte necrosis in virus-induced liver failure. J Immunol. 2010;184:466-75. (IHC-PS)
  8. 8. Suzuki-Anekoji M, Suzuki M, Kobayashi T, Sato Y, Nakayama J, Suzuki A, et al. HNK-1 glycan functions as a tumor suppressor for astrocytic tumor. J Biol Chem. 2011;286:32824-33. (IHC-PS, IP, WB, Block)
  9. 9. Yoshihara Y, Oka S, Watanabe Y, Mori K. Developmentally and spatially regulated expression of HNK-1 carbohydrate antigen on a novel phosphatidylinositol-anchored glycoprotein in rat brain. J Cell Biol. 1991;115:731-44. (WB, Purification)