Mouse IgG (H+L), Human ads Antibody : HRP

Referência OASB01516

Tamanho : 1.0mL

Marca : Aviva Systems Biology

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Goat Anti-Mouse IgG(H+L) Antibody - HRP Conjugated (OASB01516)

Datasheets/ManualsPrintable datasheet for Goat Anti-Mouse IgG(H+L) Antibody - HRP Conjugated (OASB01516)
Product Info
Tested Species ReactivityMouse
Predicted Species ReactivityMouse
ClonalityPolyclonal
IsotypeIgG
HostGoat
ConjugationHRP
ApplicationELISPOT, IHC-F, IHC-P, ICC, WB
Additional InformationDescription: Pepsin digest of Goat Anti-Mouse IgG(H+L), Human ads - Pooled antisera from goats hyperimmunized with mouse IgG; purified by affinity chromatography on mouse IgG covalently linked to agarose; reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3 and with the light chains of mouse IgM and IgA; cross-adsorbed against human immunoglobulins and pooled sera for minimal reactivity
Reconstitution and StorageStore at 2-8C
ImmunogenMouse IgG
PurificationGel filtration chromatography of pepsin digested antibody.
ConcentrationLot specific
SpecificityIgG(H+L)
CharacterizationTo insure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometry forconformance with characteristics of a standard reference reagent.
Warning- The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C.- The fluorescein (FITC) and Cyanine 5 (CY5) conjugates are supplied as 0.5 mg in 1.0 mL PBS/NaN3.Store at 2- 8 C.- The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20C.- The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20C.- The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C.- The R- phycoerythrin (R- PE) and allophycocyanin (APC) conjugates are supplied as 0.25 mg in 1.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!- The Spectral Red (SPRD) and R- phycoerythrin- Cyanine 5.5 (R- PE- CY5.5) conjugates are suppliedas 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!- Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed.
DilutionImmunofluorescence:
ELISA:
FITC conjugate
BIOT conjugate
R-PE, APC, SPRD, CY5 and
R-PE-CY5.5 conjugates
AP conjugate
HRP conjugate
BIOT conjugate
<= 1 ug/106 cells
<= 1 ug/106 cells
<= 0.1 ug/106 cells
1:2,000-1:4,000
1:4,000-1:8,000
1:5,000-1:20,000
Application InfoIndirect immunofluorescent staining in conjunction with mouse monoclonal antibodies, Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA), Western blotting, Dot-and slot-immunoblotting, Immunohistochemistry
Other Applications DataSince applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need.
Storage- The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC) and Cyanine 5 (CY 5) conjugates are supplied as 0.5 mg in 1.0 mL PBS/NaN3
Store at 2- 8 C
- The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C
- The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C
- The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) and allophycocyanin (APC) conjugates are supplied as 0.25 mg in 1.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- The Spectral Red (SPRD) and R- phycoerythrin- Cyanine 5.5 (R- PE- CY 5.5) conjugates are suppliedas 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed.
Cross AbsorptionPooled human sera and purified human paraproteins.
Gene Full NameIg gamma-1 chain C region