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> Mouse IgG2b antibody (TRITC)

Mouse IgG2b antibody (TRITC)

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Referência orb347352-1mg

Tamanho : 1mg

Marca : Biorbyt

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    Mouse IgG2b antibody (TRITC)

    Catalog Number: orb347352

    Catalog Numberorb347352
    CategoryAntibodies
    DescriptionMouse IgG2b antibody (TRITC)
    Species/HostGoat
    ClonalityPolyclonal
    Tested applicationsDOT, ELISA, FLISA, IF, IHC, WB
    ReactivityMouse
    IsotypeIgG
    ImmunogenMouse IgG2b heavy chain
    Concentration1.0 mg/mL
    Dilution rangeELISA: 1:2,000 - 1:10,000, FLISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, IF: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
    Form/AppearanceLyophilized
    PurityAnti-MOUSE IgG2b (Gamma 2b chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2b. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
    ConjugationTRITC
    StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namesgoat anti-MOUSE IgG2b Antibody rhodamine Conjugati
    NoteFor research use only
    Application notesAnti-Mouse IgG2b Rhodamine antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting as well as other anti IgG2b antibody based assays. Mouse IgG2b secondary antibody is available in a variety of formats.
    Expiration Date12 months from date of receipt.
    Mouse IgG2b antibody (TRITC)

    Dot Blot of Goat anti-Mouse IgG2b Antibody Rhodamine Conjugated Pre-absorbed. Antigen: Mouse IgG2b. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Mouse IgG2b Antibody Rhodamine Conjugated Pre-absorbed at 1:1000 overnight at 4°C. Block: orb348637 for 1 HR at RT.