NCI-H209
Marca : CLS Cell Lines Service
NCI-H209 Cells
General information
Description | The NCI-H209 cell line was derived by A.F. Gazdar and associates in 1979 from the bone marrow of a patient with small cell cancer of the lung. The bone marrow specimen was taken prior to therapy. The line is a classic SCLC cell line which expresses elevated levels of four biochemical markers (neuron specific enolase, brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity. C-myc DNA sequences are not amplified. No gross structural DNA abnormalities were detected. This is a cell line that grows as large aggregates in suspension. Only the aggregates are viable, but no meaningful viability percentage can be measured. The medium will normally contain large amounts of cell debris. The cells express an aberrant form of RB1 that is not phosphorylated, apparently due to a single point mutation at codon 706 (Cys-> Phe). |
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Organism | Human |
Tissue | Lung |
Disease | Small cell carcinoma |
Metastatic site | Bone marrow |
Synonyms | H209, H-209, NCIH209 |
Characteristics
Age | 55 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | NCI-H209 (Cytion catalog number 300183) |
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Biosafety level | 1 |
Expression / Mutation
Protein expression | p53 negative |
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Isoenzymes | G6PD, B, PGM1, 1-2, PGM3, 1, ES-D, 1, Me-2, 0, AK-1, 1, GLO-1, 1-2, Phenotype Frequency Product = 0.0624 |
Tumorigenic | Yes, forms transplantable tumors with typical SCLC histology in nude mice |
Products | The line produces normal amounts of p53 mRNA relative to normal lung. |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Subculturing | Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 2 x 10^5 cells/ml and keep the cell concentration within the range of 1 x 10^5 to 1 x 10^6 cells/ml for optimal growth. |
Split ratio | A ratio of 1:2 to 1:3 is recommended |
Seeding density | 1 x 10^5 cells/mL |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | Amelogenin: x,x CSF1PO: 11 D13S317: 11 D16S539: 9,12 D5S818: 12 D7S820: 9 TH01: 7,9 TPOX: 8 vWA: 18,19 D3S1358: 18 D21S11: 32.2 D18S51: 13 Penta E: 11,12 Penta D: 11,12 D8S1179: 12,13 FGA: 20,24 |
HLA alleles | A*: 02:01:01, 34:02:01 B*: 14:01:01, 40:01:02 C*: 03:04:01, 08:02:01 DRB1*: 04:05:01, 15:01:01G DQA1*: 01:02:01, 03:03:01 DQB1*: 03:02:01, 06:02:01 DPB1*: 03:01:01G, 04:01:01G E: 01:01:01, 01:03 |