Western Blot. Flow Cytometry: 0.5 µg/ml. Immunoprecipitation. Immunocytochemistry. Immunohistochemistry on Paraffin Sections.
Reactivity
Amphibian, Mammalian
Antibody Host
Mouse
Isotype
IgG1
Clonality
Monoclonal
Immunogen
Keratin-enriched preparation from human epidermoid carcinoma cell line A431
Specificity
The antibody reacts with Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18 (recognized epitope conserved within all species). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
Buffer
PBS, pH~7.4 State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration
lot specific
Purification
Affinity Chromatography on Protein A
Conjugation
Unconjugated
Storage
Store undiluted at 2-8°C. DO NOT FREEZE!
Stability
Shelf life: one year from despatch.
Background
Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.