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- Specifications
Product Description
Rabbit anti-mouse IgG recognizes mouse IgG F(ab')2 fragment. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with Peroxidase.
Immunogen
Mouse IgG F(ab')2 fragment
Host
Rabbit
Reactivity
Mouse
Form
Lyophilized
Conjugation
HRP
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation Note
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
Lyophilized from 5 mM sodium phosphate, 70 mM NaCl, 50% (v/v) glycerol, pH 7.4 (0.01% (w/v) gentamicin sulfate (Do NOT add sodium azide!), 10 mg/mL BSA (IgG and Protease free))
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 1 mL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. - Applications
Western Blot
Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
Dot Blot
Enzyme Immunoassay
- Publication Reference
- Immunohistochemistry with enzyme labeled antibodies: a brief review.
Farr AG, Nakane PK.
J Immunol Methods 1981 Jan; 47(2):129.
- Immunohistochemistry with enzyme labeled antibodies: a brief review.