SCLC-21H
Marca : CLS Cell Lines Service
SCLC-21H Cells
General information
Organism | Human |
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Tissue | Lung |
Disease | Carcinoma |
Metastatic site | Pleural effusion |
Synonyms | SCLC21H |
Characteristics
Age | 46 years |
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Gender | Male |
Ethnicity | Caucasian |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | SCLC-21H (Cytion catalog number 300225) |
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Biosafety level | 1 |
Expression / Mutation
Oncogenes | myc amplification present, c-myc expression high |
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Tumorigenic | Yes in nude mice |
Ploidy status | Aneuploid |
Karyotype | Modal chromosome number 42/43, range 39-44. Chromosome deletion 3p. |
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 45 hours |
Subculturing | Once or twice a week add 5 ml of fresh cell culture medium, as soon as the culture medium gets acidic. Suculture as soon as many very large clusters are observed. Dissociate the clusters by collecting the cells, rinsing once using PBS without calcium/magnesium and adding 3-5 ml Accutase. Incubate for 10minutes at 37 degree Celsius. Collect the cells following centrigation, resuspend in fresh cell culture medium and count. |
Split ratio | A ratio of 1:2 to 1:4 is recommended |
Seeding density | 2 to 4 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | Cells will recover from freezing within 24 to 48 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | CSF1PO: 10 D13S317: 12 D16S539: 12 D5S818: 11,12 D7S820: 11 TH01: 9.3 TPOX: 8,9 vWA: 17 D3S1358: 15 D21S11: 29,31.2 D18S51: 14,15 Penta E: 12,13 Penta D: 9 D8S1179: 12,13 FGA: 22 |