SNAP 25 (Synaptosomal-associated Protein 25, SNAP-25, bA416N4.2, dJ1068F16.2, FLJ23079, HGNC:11132, Resistance to Inhibitors of Cholinesterase 4 Homolog, RIC4, RIC-4, SEC9, SNP25, Super Protein, SUP) (Biotin)

Referência S1014-70F-Biotin-100ul

Tamanho : 100ul

Marca : US Biological

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S1014-70F-Biotin Rabbit Anti-SNAP 25 (Synaptosomal-associated Protein 25, SNAP-25, bA416N4.2, dJ1068F16.2, FLJ23079, HGNC:11132, Resistance to Inhibitors of Cholinesterase 4 Homolog, RIC4, RIC-4, SEC9, SNP25, Super Protein, SUP) (Biotin)

Clone Type
Polyclonal
Host
mouse
Source
human
Swiss Prot
P60880
Isotype
IgG1
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hm Hu Po Rt
Accession #
NP_001266
Shipping Temp
Blue Ice
Storage Temp
-20°C

Research studies have used this antibody to study the distribution of synaptic changes in the hippocampus of patients with medically refractory temporal lobe epilepsy (1, 3, 8). ||Applications:|Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. ||Recommended Dilutions: |Immunohistochemistry (Paraffin): Does not require protein digestion or antigen retrieval using heat treatment prior to staining of paraffin sections. |Optimal working dilutions to be determined by researcher.||IHC Positive Control:|Brain||Hybridoma:|NSO myeloma cells with spleen cells from Balb/c mice. ||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 3H2886 (SP12)|Host: mouse|Source: human|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.|Purity: Purified by Protein A affinity chromatography from tissue culture supernatent|Immunogen: Crude human synaptic immunoprecipitate.|Specificity: Recognizes human SNAP-25; an antigen of 26-27kD molecular weight. Will react with SNAP-25 from COS cells, but not with the fusion protein from bacterial systems. Species Crossreactivity: rat, hamster, gerbil and porcine||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Crude human synaptic immunoprecipitate.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Purity
Purified by Protein A affinity chromatography from tissue culture supernatent
Specificity
Recognizes human SNAP-25; an antigen of 26-27kD molecular weight. Will react with SNAP-25 from COS cells, but not with the fusion protein from bacterial systems. Species Crossreactivity: rat, hamster, gerbil and porcine
References
1. Honer, W.G., et al., 1994, Hippocampal synaptic pathology in patients with temporal lobe epilepsy. Acta Neuropathol. 87: 202-210. 2. Honer, W.G., et al., 1992, Regional synaptic pathology in Alzheimer's disease. Neurobiol. Aging. 13: 375-382. 3. Honer, W.G., et al., 1993, Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex. Brain Res. 609: 9-20. 4. Honer, W.G., et al., 1992, Characterization of a synaptic antigen of interest in neuropsychiatric illness. Biol. Phychiatry 31: 147-158. 5. Honer, W.G., et al., 1989, Monoclonal antibodies to study the brain in schizophrenia. Brain Res. 500: 379-383. 6. Masliah, E., et al., 1994, Topographical distribution of synaptic-associated proteins in the neuritic plaques of Alzheimer's disease hippocampus. Acta Neuropathol. 87: 135-142. 7. Simpson, I.A., et al., 1994, Decreased concentrations of GLUT1 and GLUT3 glucose transporters in the brains of patients with Alzheimer's disease. Ann Neurol. 35: 546-551. 8. Wakabayashi, K., et al., 1994, Synapse alterations in the hippocampalentorhinal formation in Alzheimer's disease with and without Lewy body disease. Brain Research 667: 24-32. 9. Dickson, D.W., et al., 1994, Hippocampal sclerosis: a common pathological feature of dementia in very old (>80 years of age) humans. Acta Neuropathologica 88: 212-221. 10. Dickson, D.W., et al., 1995, Correlations of synaptic markers with cognitive status in prospectively studied elderly humans. Neurobiol Aging (in press).