OKEH00569 | TLR4 ELISA Kit (Rat) Clinisciences

Referência OKEH00569

Tamanho : 96Wells

Contactar o distribuidor local :

Telefone : +1 850 650 7790

Marca : Aviva Systems Biology

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Contactar o distribuidor local :

Telefone : +1 850 650 7790

Datasheets/Manuals	Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA Datasheet	Printable COA datasheet for OKEH00569

Product Info

Predicted Species Reactivity

Rat, Rattus norvegicus

Application

ELISA-Sandwich

ELISA Kit Detection Method

Colorimetric, OD450 nm

ELISA Kit Duration

~ 3 Hours

ELISA Kit Principle

Aviva Systems Biology TLR4 ELISA Kit (Rat) (OKEH00569) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TLR4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TLR4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TLR4 captured in well.

ELISA Kit Range

0.312-20ng/mL

ELISA Kit Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

ELISA Kit Reproducibility

Mean Intra-assay CV%: < 4.8% (n = 20)
Mean Inter-assay CV%: < 8.1% (n = 20)

ELISA Kit Component

Component	Amount
Anti-TLR4 Microplate	96 Wells (12 x 8 Well strips)
TLR4 Lyophilized Standard	2
Sample Diluent	1 x 20 mL
100X Biotinylated TLR4 Detector Antibody	1 x 120 uL
100X Avidin-HRP Conjugate	1 x 120 uL
Detector Antibody Diluent	1 x 12 mL
Conjugate Diluent	1 x 12 mL
25X Wash Buffer	1 x 30 mL
TMB Substrate	1 x 10 mL
Stop Solution	1 x 10 mL

Reconstitution and Storage

Store as indicated in product manual.

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Sensitivity

0.159 ng/mL

Specificity

Natural and recombinant Rat Toll-like receptor 4

Assay Info

Assay Methodology: Quantitative Sandwich ELISA

Gene Symbol	Tlr4
Gene Full Name	toll-like receptor 4
Alias Symbols	toll4;toll-like receptor 4.
NCBI Gene Id	29260
Protein Name	Q9QX05
Description of Target	Toll-like receptor 4
Uniprot ID	https://www.uniprot.org/uniprot/Q9QX05
Protein Accession #	NP_003257.1
Nucleotide Accession #	NM_003266.3
Protein Size (# AA)	839
Molecular Weight	95 kDa

Biological pathways

Name	# of Products
Activation of MAPK activity	317
Cellular response to mechanical stimulus	218
Defense response to Gram-negative bacterium	93
Detection of fungus	14
Detection of lipopolysaccharide	19
I-kappaB phosphorylation	65
Innate immune response	905
Intestinal epithelial structure maintenance	20
MyD88-dependent toll-like receptor signaling pathway	331
MyD88-independent toll-like receptor signaling pathway	288
Negative regulation of ERK1 and ERK2 cascade	59
Negative regulation of interferon-gamma production	55
Negative regulation of interleukin-17 production	68
Negative regulation of interleukin-23 production	16
Negative regulation of interleukin-6 production	98
Negative regulation of osteoclast differentiation	65
Negative regulation of tumor necrosis factor production	93
Positive regulation of chemokine production	128
Positive regulation of inflammatory response	172
Positive regulation of interferon-alpha production	52
Positive regulation of interferon-beta production	86
Positive regulation of interferon-gamma production	108
Positive regulation of interleukin-1 production	16
Positive regulation of interleukin-10 production	69
Positive regulation of interleukin-12 biosynthetic process	28
Positive regulation of interleukin-12 production	171
Positive regulation of interleukin-6 production	149
Positive regulation of interleukin-8 biosynthetic process	38
Positive regulation of interleukin-8 production	127
Positive regulation of NF-kappaB import into nucleus	114
Positive regulation of NF-kappaB transcription factor activity	346
Positive regulation of nitric-oxide synthase biosynthetic process	110
Positive regulation of platelet activation	30
Positive regulation of transcription from RNA polymerase II promoter	1210
Positive regulation of tumor necrosis factor biosynthetic process	63
Positive regulation of tumor necrosis factor production	168
T-helper 1 type immune response	37
Toll signaling pathway	369
Toll-like receptor 1 signaling pathway	309
Toll-like receptor 2 signaling pathway	309
Toll-like receptor 4 signaling pathway	330

ELISA Titer: 1:1562500
Positive Control: HepG2 cell lysate" data-size="300x300" class="product-image-wrapper cover">

TLR4 Antibody - middle region (ARP59041_P050)
Catalog #: ARP59041_P050

Species Tested: Human

Application: IHC, WB

Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
ELISA Titer: 1:312500
Positive Control: A549 cell lysate" data-size="300x300" class="product-image-wrapper cover">

TLR4 Antibody - C-terminal region (ARP59160_P050)
Catalog #: ARP59160_P050

Species Tested: Human

Application: WB

Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
TLR4 ELISA Kit (Human) : 96 Wells (OKEH02932)
Catalog #: OKEH02932

Application: ELISA-Sandwich

Kit Range: 0.625-40ng/mL

Sensitivity: 0.156 ng/mL

Size: 96T
Tlr4 ELISA Kit (Mouse) : 96 Wells (OKEH02933)
Catalog #: OKEH02933

Application: ELISA-Sandwich

Kit Range: 0.312-20ng/mL

Sensitivity: 0.158 ng/mL

Size: 96T
TLR4 Recombinant Protein (OPCD07498)
Catalog #: OPCD07498

Conjugation: Unconjugated

Application: Ctrl (+), SDS-PAGE, WB

Format: Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

TLR4 ELISA Kit (Rat)

Referência OKEH00569

Contactar o distribuidor local :

Marca : Aviva Systems Biology

Contactar o distribuidor local :

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