Trans10 Chemically Competent Cell
Referência CD101-02
Tamanho : 20×100ul
Marca : TransGen Biotech
Trans10 Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 108 cfu/μg DNA (tested by pUC19 plasmid DNA). The competent cell is resistant to StrR.
Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80 lacZΔM15Δ lacX74 recA1 araΔ139Δ(ara-leu)7697 galU galKrpsL (StrR) endA1 nupG
Features
• High transformation efficiency: >108 cfu/μg ( pUC19 DNA).
• StrR.
• Blue/white selection.
• Toxic gene cloning and stable replication of plasmid DNA.
Storage
at -70 °C for six months
Shipping
Dry ice (-70℃)
Wang X1, Xuan Y1, Han Y, er al. Regulation of HIV-1 Gag-Pol expression by Shiftless, an inhibitor of programmed -1 ribosomal frameshifting[J]. Cell, 2019, 24, 176(3):625-635.
Wang Y , Shi M , Feng H , et al. Structural Insights into Non-canonical Ubiquitination Catalyzed by SidE[J]. Cell, 2018,17, 173(5):1231-1243.
Guo Z , Kang S , Chen D , et al. MAPK Signaling Pathway Alters Expression of Midgut ALP and ABCC Genes and Causes Resistance to Bacillus thuringiensis Cry1Ac Toxin in Diamondback Moth[J]. PLOS Genetics, 2015, 11(4):e1005124.
Chen X , Xu F , Zhu C , et al. Dual sgRNA-directed gene knockout using CRISPR/Cas9 technology in Caenorhabditis elegans[J]. Scientific Reports, 2014, 4:7581.
Dai Z , Wang B , Liu Y , et al. Producing aglycons of ginsenosides in bakers' yeast[J]. Scientific Reports, 2014, 15(4):3698.