TransStart® FastPfu DNA Polymerase (with 2.5 mM dNTPs)
Referência AP221-13
Tamanho : 6×500units
Marca : TransGen Biotech
Contactar o distribuidor local :
Telefone : +1 850 650 7790
ManualTransStart® FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase.
• Extension rate is about 2-4 kb/min.
• TransStart® FastPfu DNA Polymerase offers 54-fold fidelity as compared to EasyTaq® DNA Polymerase.
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.
Applications
• High fidelity PCR
• High yield and fast PCR
• Blunt end cloning
• Site-directed mutagenesis
• Complex templates
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
| Component | AP221-01/11 | AP221-02/12 | AP221-03/13 |
| TransStart® FastPfu DNA Polymerase | 250 U×1 | 500 U×1 | 500 U×6 |
| 5×TransStart® FastPfu Buffer | 1.2 ml×1 | 1.2 ml ×2 | 1.2 ml ×12 |
| 2.5 mM dNTPs | - / 500 μl×1 | - / 1 ml ×1 | - / 1 ml ×6 |
| 50 mM MgSO4 | 200 μl×1 | 400 μl ×1 | 1 ml ×1 |
| PCR Stimulant | 200 μl×1 | 400 μl×1 | 1 ml ×1 |
| 6×DNA Loading Buffer | 200 μl×1 | 1 ml ×1 | 1 ml ×2 |
Zhang F , Tang W , Hedtke B , et al. Tetrapyrrole biosynthetic enzyme protoporphyrinogen IX oxidase 1 is required for plastid RNA editing[J]. Proceedings of the National Academy of Sciences, 2014, 111(5):2023-2028.
Liang X , Dong X , Moerman D , et al. Sarcomeres Pattern Proprioceptive Sensory Dendritic Endings through UNC-52/Perlecan in C.elegans[J]. Developmental Cell, 2015, 33(4):388-400.
Huang X S , Wang W , Zhang Q , et al. A Basic Helix-Loop-Helix Transcription Factor, PtrbHLH, of Poncirus trifoliata Confers Cold Tolerance and Modulates Peroxidase-Mediated Scavenging of Hydrogen Peroxide[J]. PLANT PHYSIOLOGY, 2013, 162(2):1178-1194.
Ma Y , Li B , Wang C , et al. 5-HTTLPR Polymorphism Modulates Neural Mechanisms of Negative Self-Reflection[J]. Cerebral Cortex, 2014, 24(9):2421-2429. Cao Y X , Xiao W H , Liu D , et al. Biosynthesis of odd-chain fatty alcohols in Escherichia coli[J]. Metabolic Engineering, 2015, 29:113-123.