Canvax Biotech
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Canvax is a leading manufacturer and supplier of the most innovative solutions, kits and R&D Reagents inside Molecular Biology fields. Since its foundation in 2001, Canvax offers reliable, cost-effective and easy-to-use innovative tools for research. Based in Córdoba (Spain), since its beginning it has focused on R&D of multiplex high throughput platforms (HTS) for Drug discovery and Diagnostic applied biosensors. Over a decade later, Canvax is a worldwide leading expert in GPCR expression in heterologous cells, with important patents and exclusive know-how. Canvax prides itself to be the first company to get an unprecedented milestone that could revolutionize the Diagnostics sector: Canvax established stable high-level expression of odour GPCRs into heterologous cell lines in 2014. This key milestone, that could be applied in diagnostic, perfume and cosmetic industry, are being employed to obtain a molecular nose, a sensitive and non-invasive diagnostic molecular device for early detection of cancer, as the nose of trained dogs do. Thanks to this project, Canvax was awarded, in 2013, with the largest Public & Private Contract for Pre competitive Technology Development in Spanish history. Website : www.lifescience.canvaxbiotech.com |
![SYBR Green Mix](https://www.clinisciences.com/pt/upload/thumbs/sybr-qpcr-vh4anx-40a664.jpg)
SYBR Green Mix
SYBR Green
is an aromatic organic compound of chemical formula C32H37N4S belonging to the
asymmetric cyanines (fluorophores). It has the ability to bind to nucleic acids
and emit fluorescence. It is therefore used in molecular biology as a
fluorescent agent, especially when carrying out quantitative PCRs.
SYBR Green
is the most commonly used dye for non-specific detection. SYBR green I is a
molecule that can bind to all types of double stranded nucleic acids. It is not
an intercalating agent: by definition, an intercalating agent is a molecule
that can be inserted between the trays formed by the paired bases of a nucleic
acid; The SYBR green does not meet this definition. Once fixed, it becomes a
very good fluorophore. Thus, the double-stranded DNA / SYBR green I complex
absorbs blue light (λmax = 497 nm) and emits green light (λmax = 520 nm). The
emitted fluorescence can be detected and related to the amount of target.
Some real-time PCR instruments require an internal reference dye for fluorescent signal normalization and correction of optical well-to-well variations. The variation in fluorescence may result from the design of the instrument. The ROX Passive Reference Dye is an inert additive that provides a constant fluorescent signal for standardization of the sample throughout the qPCR test in real time.
Universal | All instruments |
Without ROX | Bio-Rad
CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon®, Opticon 2,
Chromo4; Cepheid SmartCycler®; Eppendorf Mastercycler® ep realplex, realplex 2 s; Illumina Eco qPCR; Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000; Roche Applied Science LightCyclerTM 480; Thermo Scientific PikoReal Cycler. |
Low ROX | Applied
Biosystems 7500, 7500 Fast, ViiA7; Stratagene MX4000, MX3005P, MX3000P |
High ROX | Applied
Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne, StepOnePlus |