Hams F12 medium emerged initially to address the specific requirements of near diploid cell cultures, offering a robust enhancement over its precursor, F10 medium. Engineered primarily for the cloning and successive propagation of CHD-3 and CHL-1 cell lines sans serum supplementation, its adaptability extends to fostering optimal growth conditions for cells even at sparse densities.

Distinguished by elevated concentrations of choline, I-inositol, putrescine, and a spectrum of essential amino acids, F-12 medium presents a fertile ground for the proliferation of various cell types. Its efficacy spans the cultivation of carcinoma cells, rat skeletal myoblasts, lung cells, as well as embryonic tissues from rats, rabbits, or chickens.

Hams F12 stands as a pivotal tool in cell culture research, offering tailored support for a diverse array of cell lines and experimental needs.