SYBR Green Mixes without ROX for RT-qPCR
Mix for quantitative RT-PCR SYBR Green without ROX are made for use with qPCR devices that do not require an internal reference dye for fluorescent signal normalization and correction of optical well-to-well variations.
The SYBR Green mix consists of a Reverse transcriptase, a Taq polymerase, dNTPs, MgCl2 and SYBR Green.
It is possible to add ROX dye to these mixes for use with qPCR devices requiring the presence of ROX.
Here's how to select the appropriate ROX dye for the qPCR device you are using:
| Universal | All instruments |
| Without ROX | Bio-Rad
CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon®, Opticon 2,
Chromo4; Cepheid SmartCycler®; Eppendorf Mastercycler® ep realplex, realplex 2 s; Illumina Eco qPCR; Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000; Roche Applied Science LightCyclerTM 480; Thermo Scientific PikoReal Cycler. |
| Low ROX | Applied
Biosystems 7500, 7500 Fast, ViiA7; Stratagene MX4000, MX3005P, MX3000P |
| High ROX | Applied
Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne, StepOnePlus |
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