HRP Chromogen for IHC - DAB
DAB (3,3'-Diaminobenzidine) is the most widely used chromogen for use with HRP. DAB substrate forms a brown colored deposit when it reacts with peroxidase in the presence of hemoglobin.
The use of DAB is simple, simply incubate the slides for 5 minutes in a humid chamber at room temperature. The appearance of the staining can be followed by observation under the microscope.
When staining is complete, it is necessary to rinse the slides with water for 1-2 minutes to remove excess DAB. The precipitate from DAB is insoluble in alcohol and other organic solvents. Samples can be dehydrated and slides mounted permanently (O-Mount).
Name
DAB (3,3'-Diaminobenzidine)
Applications
IHC, ICC, ISH, WB, ELISA
Detection enzyme
HRP
Reaction type
Precipitating Reaction
Precipitate color
Brown
Microscopy
Brightfield, Darkfield, Electron, Spectral Imaging
Mounting
Aqueous (Hardening), Non-Aqueous (Permanent)
Other properties
Heat Resistant, Multiple Labeling
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RUOCE / IVD
- Unconjugated 1056
- HRP 100
- FITC 66
- Biotin 65
- PE 52
- APC 47
- MaxLight 490 29
- MaxLight 550 29
- MaxLight 650 29
- MaxLight 405 18
- MaxLight 750 18
- Cy3 14
- Alexa Fluor 350 8
- Alexa Fluor 488 8
- Alexa Fluor 555 8
- Alexa Fluor 750 8
- Cy5 8
- Cy5.5 8
- Cy7 8
- APC-CY5.5 1
- APC-Cy7 1
- Alexa Fluor 405 1
- Alexa Fluor 594 1
- Alexa Fluor 647 1
- Alexa Fluor 680 1
- PE-Cy5 1
- PE-Cy5.5 1
- PE-Cy7 1
- PerCP-Cy5.5 1
- PerCp 1
- RBITC 1
- human 1070
- mouse 333
- rat 309
- e. coli 53
- cow 50
- dog 47
- pig 47
- cat 26
- rabbit 24
- horse 23
- monkey 20
- sheep 18
- camel 16
- hamster 16
- guinea pig 7
- chicken 6
- plant 5
- donkey 4
- duck 4
- fish 4
- goose 4
- pigeon 4
- monosan dab high contrast 2
- xenopus 2
- zebrafish 2
- 1 1
- monosan plus (hrp) broad spectrum (dab)kit 1
- Primary antibody 1195
- Biochemicals 1157
- Protein/peptide 990
- ELISA/ELISPOT 360
- Inhibitor/Antagonist/Agonist 277
- Buffers and reagents 155
- CRISPR/Cas9 149
- RNAi 102
- Probe 62
- Destination vector 50
- kit 38
- Other products 36
- Secondary antibody 13
- Cell line 6
- cDNA Clone 4
- Lysate 2
- Paraffin tissue section 1
- Primer 1
- rabbit 581
- mouse 440
- human 351
- e. coli 146
- yeast 122
- 48
- rat 40
- goat 36
- horse 12
- dog 10
- hamster 10
- sheep 10
- virus 9
- cow 8
- monkey 8
- pig 8
- bird 5
- chicken 5
- cat 1
- donkey 1
- guinea pig 1
- ELISA 742
- WB 713
- IHC 437
- FLISA 109
- IP 100
- ICC 90
- FC 86
- IF 80
- Enzyme-linked immunosorbent assay for Antigen Detection. 10
- 0.078 ng/mL 3
- Chemiluminescence 3
- 0.1 ng/mL 2
- 0.116 ng/ml 1
- ISH 1
- PCR 1
- Polyclonal 619
- 2G6 31
- 4G5 31
- 1F8 30
- 1G1 30
- 1G5 30
- 10i46 25
- Monoclonal 22
- 13H261 21
- 3H1166 21
- M-A261 12
- HLy9.1.25 8
- 1626CT702.263.21 6
- 1A10A10 5
- 10.1F8 4
- 12H6.D3 4
- 13.1G11 4
- 13.2G8 4
- 13.6D9 4
- 13.7E2 4
- 13.7H4 4
- 13.8E5 4
- 13B4.E10 4
- 14.2H9 4
- 14F10.H9 4
- 1A6 4
- 26.7C4 4
- 2E4 4
- 3G8 4
- 4A8 4
- 4B9 4
- 5A11 4
- 5A2 4
- 6.1H3.D9.C6 4
- 6.1H8.F9.G6 4
- 6.2D2.E1.D11 4
- 6.7A2.A4.D3 4
- 6.8A2.B8.A11 4
- 6.8E7.A7.A1 4
- 8C7 4
- 8G1 4
- 8H12 4
- 8H6 4
- 9.21C1.G1.F1.A5 4
- 9.22E1.F5.H10 4
- 9.24E10.F3.H12 4
- IgG.2A5 4
- IgG.2E9 4
- Not Applicable 4
- RB21227 4
- 6D23 3
- SAA1697 3
- 12K274 2
- 17D17 2
- 17L18 2
- 202.36 2
- 2213 2
- 2M2 2
- 3F9-2C2 2
- 5B20 2
- 7H111 2
- 8C10 2
- B2M-01 2
- B2M-02 2
- 11C257 1
- 12K99 1
- 134-2C2 1
- 13B822 1
- 5K78 1
- 5K78 (134-2C2) 1
- 6C1 1
- 6C5 1
- 9H177 1
- ADAB-8 1
- DAB-6 1
- Recombinant 1
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