Preparation of DNA libraries for Illumina NGS

There are different technologies for Next Generation Sequencing (NGS). The most widespread at present is the Illumina technology, which generates more than 90% of the sequencing data worldwide. The Illumina sequencing technology is based on the principle of Sequencing by synthesis (SBS). Sequencing by synthesis consists of the addition of a fluorescent nucleotide as each dNTP is added and then cleaved to allow the incorporation of the next base.

As sequencing is performed on DNA fragments, obtained from a sample and representing a library, each fragment ends with a fluorescent base. The end result is true base-by-base sequencing that provides accurate data for a wide range of applications.

In order for Illumina sequencing to be as accurate as possible, it is essential to obtain a quality DNA library. To this end, we offer a complete range of products for the preparation of DNA libraries for the Illumina NGS.