New Ultra-Sensitive qPCR Gene Mutation Detection Kits

QClamp® Gene Mutation Detection Kit is a highly sensitive qPCR assay that provides detection sensitivity of 0.1%or lower. It is ideal for screening rare and actionable somatic mutations in oncogenes. The assay utilizes a sequence-specific clamp (Xeno-Nucleic Acid probe) that suppresses PCR amplification of wild-type DNA template and selectively amplifies only mutant template. The kits provide a rapid, reproducible and affordable solution which employs a simple workflow and PCR machines that are commonly used in research and clinical labs.

 

 

 

DiaCarta scientists have developed innovative new nucleic acid molecular oligomers that hybridize by Watson-Crick base pairing to target DNA sequences yet have a modified chemical backbone. The xenonucleic acid oligomers (figure: Watson-Crick Base Pairing of DNA with cognate XNA) are highly effective at hybridizing to target sequences and can be employed as molecular clamps in quantitative real-time polymerase chain reactions (PCR) or as highly specific molecular probes for detection of nucleic acid target sequences.

 

 

 

QClamp® is a revolutionary new way to screen for somatic mutations that utilize a sequence-specific clamp that suppresses PCR amplification of wild type template DNA. QClamp® allows selective PCR amplification of only mutant templates, which allows the detection of mutant DNA in the presence of a large excess of wild-type templates from a variety of samples including FFPE, liquid biopsy, and traditionally challenging cytology samples.