Construction of complex vectors
Vectors using standard backbones, but which contain complex components that need to be created
These are vectors using standard backbones, but which contain complex components that need to be created. They typically require multiple steps to build, and often involve complicated procedures such as amplification of large fragments from template DNA, fusion of multiple fragments, de novo gene synthesis, and targeted mutagenesis.
Additional turnaround time for complex vectors:
Cloning procedure
Aditionnal turnaround*
Insertion of one PCR fragment (≤6 kb)
10-15 days
Insertion of two fused PCR fragments (≤6 kb)
10-15 days
Insertion of three fused PCR fragments (≤6 kb)
11-18 days
Insertion of four fused PCR fragments (≤6 kb)
12-20 days
Insertion of five fused PCR fragments (≤6 kb)
11-26 days
De novo gene synthesis (≤1.2 kb)
13-16 days
De novo gene synthesis (1.2-2.4 kb)
16-20 days
De novo gene synthesis (2.4-3.6 kb)
16-22 days
De novo gene synthesis (3.6-5 kb)
>20 days
Mutagenesis
14-16 days
* Processing fee and longer turnaround may apply when the fragment contains regions that are difficult to synthesize such as high GC content, simple repeats or segmental repeats.