TransGen Biotech
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TransGen Biotech, Inc. is a researcher, developer, manufacturer and distributor of more than 200 molecular and cellular biology products and kits for life science research and molecular diagnostics. In 2001, the company was founded by three scientists with a mission to produce innovative and cost-effective products for life science research. Currently, our products cover: plasmid based DNA markers, high efficiency chemically competent cells, 5 minutes PCR product cloning and expression vectors, a variety of PCR enzymes and supermix, RNase H deficient and high temperature RT enzymes, qPCR and qRT-PCR supermix, the highest efficiency mutagenesis kits, high quality nucleic acid extraction and purification kits, unstained and prestained protein markers, western blot markers, and protein purification resins, cell culture and transfection reagents, antibodies.
Website : www.transgenbiotech.com
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Competent cells
Cellular competence refers to a cell's ability to absorb foreign (extracellular) DNA from its environment. The process of genetic absorption is called transformation. In some cases, the genetic material absorbed by a cell may be incorporated, or recombined, into its own genome. This incorporation of genetic material into the host genome is called horizontal gene transfer (HGT) or lateral gene transfer.
Natural competence and gene transfer have facilitated many adaptations in prokaryotic and eukaryotic cells. Competent cells can either occur naturally or be made competent artificially.
There are two types of artificially competent cells: chemically competent cells and electro-competent cells. Chemically competent cells are cells that have been made competent by a salt treatment followed by a heat shock step. Electro-competent cells are made competent by using an electrical impulse from an electroporator to create temporary pores (poration) in the cell membrane of prokaryotic or eukaryotic cells.
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