Anti-EGFR antibody (1021-1210)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:2000 IF/ICC 1:50-1:200 IP 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	EGFR
Gene ID:	1956
Uniprot ID:	EGFR_HUMAN
Immunogen Region:	1021-1210
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2).
Immunogen Sequence:	QGFFSSPSTSRTPLLSSLSA TSNNSTVACIDRNGLQSCPI KEDSFLQRYSSDPTGALTED SIDDTFLPVPEYINQSVPKR PAGSVQNPVYHNQPLNPAPS RDPHYQDPHSTAVGNPEYLN TVQPTCVNSTFDSPAHWAQK GSHQISLDNPDYQQDFFPKE AKPNGIFKGSTAENAEYLRV APQSSEFIGA

Tissue Specificity	Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
Post Translational Modifications	Phosphorylated on Tyr residues in response to EGF. Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2. Monoubiquitinated and polyubiquitinated upon EGF stimulation.which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126. Ubiquitinated by ZNRF1 or CBL at different lysines in response to EGF stimulation.leading to recruitment of the ESCRT machinery and subsequent degradation in the lysosomes. Deubiquitinated by UCHL1 leading to the inhibition of its degradation. Palmitoylated on Cys residues by ZDHHC20. Palmitoylation inhibits internalization after ligand binding, and increases the persistence of tyrosine-phosphorylated EGFR at the cell membrane. Palmitoylation increases the amplitude and duration of EGFR signaling. Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.
Function	Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration. Plays a role in enhancing learning and memory performance. Plays a role in mammalian pain signaling (long-lasting hypersensitivity). Isoform 2 may act as an antagonist of EGF action. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins.
Protein Name	Epidermal Growth Factor Receptor Proto-Oncogene C-Erbb-1 Receptor Tyrosine-Protein Kinase Erbb-1
Database Links	Reactome: R-HSA-1227986 Reactome: R-HSA-1236382 Reactome: R-HSA-1236394 Reactome: R-HSA-1250196 Reactome: R-HSA-1251932 Reactome: R-HSA-1257604 Reactome: R-HSA-177929 Reactome: R-HSA-179812 Reactome: R-HSA-180292 Reactome: R-HSA-180336 Reactome: R-HSA-182971 Reactome: R-HSA-1963640 Reactome: R-HSA-1963642 Reactome: R-HSA-212718 Reactome: R-HSA-2179392 Reactome: R-HSA-2219530 Reactome: R-HSA-445144 Reactome: R-HSA-5637810 Reactome: R-HSA-5638303 Reactome: R-HSA-5673001 Reactome: R-HSA-6785631 Reactome: R-HSA-6811558 Reactome: R-HSA-8847993 Reactome: R-HSA-8856825 Reactome: R-HSA-8856828 Reactome: R-HSA-8857538 Reactome: R-HSA-8863795 Reactome: R-HSA-8866910 Reactome: R-HSA-9009391 Reactome: R-HSA-9013507 Reactome: R-HSA-9609690 Reactome: R-HSA-9634638 Reactome: R-HSA-9664565 Reactome: R-HSA-9665348 Reactome: R-HSA-9665686
Cellular Localisation	Cell Membrane Single-Pass Type I Membrane Protein Endoplasmic Reticulum Membrane Golgi Apparatus Membrane Nucleus Membrane Endosome Endosome Membrane Nucleus In Response To Egf Translocated From The Cell Membrane To The Nucleus Via Golgi And Er Endocytosed Upon Activation By Ligand Colocalized With Gper1 In The Nucleus Of Estrogen Agonist-Induced Cancer-Associated Fibroblasts (Caf) Isoform 2: Secreted
Alternative Antibody Names	Anti-Epidermal Growth Factor Receptor antibody Anti-Proto-Oncogene C-Erbb-1 antibody Anti-Receptor Tyrosine-Protein Kinase Erbb-1 antibody Anti-EGFR antibody Anti-ERBB antibody Anti-ERBB1 antibody Anti-HER1 antibody

Information sourced from Uniprot.org

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