beta Tubulin Monoclonal Antibody
Referência E-AB-20093-120
Tamanho : 120uL
Marca : Elabscience
Verified Samples | Verified Samples in WB: Hela, Mouse brain, Rat brain Verified Samples in IF: Hela |
Dilution | WB 1:5000-10000, IF 1:100-200 |
Isotype | IgG |
Host | Mouse |
Reactivity | Human, Mouse, Rat |
Applications | WB, IF |
Clonality | Monoclonal |
Immunogen | Synthetic Peptide |
Abbre | β-tubulin |
Synonyms | CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, M(beta)3, M(beta)6, MC1R, Neuron specific beta III Tubulin, Neuron-specific class III beta-tubulin, QccE-11995, QccE-15186, TBB3, TUBB3, TUBB4, Tubb 3, Tubulin, Tubulin beta 3, Tubulin beta 3 chain, Tubulin beta 4, beta 3 tubulin, beta-4 |
Swissprot | |
Calculated MW | 50 kDa |
Observed MW | 55 kDa The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Cytoplasm>cytoskeleton. |
Tissue Specificity | Expression is primarily restricted to central and peripheral nervous system. |
Concentration | 1 mg/mL |
Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
Purification Method | Protein A purification |
Research Areas | Cancer, Neuroscience, Signal Transduction, Tags and Cell Markers |
Clone No. | 4A6 |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
background | TUBB3,the class III β tubulin or Tuj1,is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms. |
Other Clones
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Unconjugated
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