BMP4 ELISA Kit (Rat)

Referência OKEH03998

Tamanho : 96Wells

Marca : Aviva Systems Biology

Solicitar mais informações

Contactar o distribuidor local :


Telefone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH03998
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Bmp4 ELISA Kit (Rat) (OKEH03998) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bmp4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Bmp4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Bmp4 captured in the well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.4% (n = 20)
Mean Inter-assay CV%: <= 9.5% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Bmp4 Microplate96 Wells (12 x 8 Well Strips)
Bmp4 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
Bmp4 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity39 pg/mL
SpecificityNatural and recombinant Rat Bone morphogenetic protein 4
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolBMP4
Gene Full Namebone morphogenetic protein 4
Alias SymbolsBone morphogenetic protein 2B.
NCBI Gene Id25296
Protein NameQ06826
Description of TargetBone morphogenetic protein 4
Uniprot IDhttps://www.uniprot.org/uniprot/Q06826
Protein Accession #NP_036959.2
Nucleotide Accession #NM_012827.2
Protein Size (# AA)408
Molecular Weight47 kDa

Você também pode estar interessado nos seguintes produtos:



Referência
Descrição
Cond.
Price Bef. VAT
orb410874-96
 96t