BRD4 TR-FRET, BioAssay™ Kit (BD1+BD2, Bromodomain, HUNK1, MCAP)

The BRD4 (BD1+BD2) TR-FRET BioAssay™ Kit is designed to measure the inhibition of BRD4 (BD1+ BD2) binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BRD4, substrate, and an inhibitor is incubated for 120 minutes. Then, the fluorescence intensity is measured using a fluorescence reader.||Applications: |Suitable for use in screening small molecular inhibitors for drug discovery and HTS applications. ||Kit Components:|298369A: Microtiter plate, 1x 1 plate|*298369B: BRD4 (BD1+BD2), 1x10ug |*298369C: BET Bromodomain Ligand, 1x50ul |*298369D: Non-acetylated Ligand 1, 1x15ul |**298369E: Tb-labeled donor, 2x10ul |**298369F: Dye-labeled acceptor, 2x10ul |**298369G: BRD TR-FRET Assay Buffer 1, 3X, 1x4ml ||Storage and Stability:|Store *298369B, *298369C and *298369D at -70°C. Store **298369E, **298369F and **298369G at -20°C. 298369A can be stored at RT. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.