Cellulase Onozuka R-10^® is used for the degradation of plant cell walls, usually for the production and isolation of metabolically active protoplasts, often in combination with Macerozyme R-10 (cat. no. 28032) (2).
The cellulase Onozuka R-10^® is a multi-component enzyme system (1). Although the preparation has high cellulase activity, it contains hemicellulases and degrades mannans, xylans, galactomannans, pectins and other polysaccharides.
Temperature optimum: 40 - 50 °C
pH-optimum: pH 4 - 5

Unit definition: 1 U catalyzes the liberation of 1 µmole glucose from sodium carboxymethyl cellulose per minute at 40 °C, pH 4.5; glucose determined with alkaline copper reagent (3).

Extraneous activities: α-amylase ca. 0.8 U, pectinase ca. 0.4 U, protease ca. 0.01 DMC-U, hemicellulase ca. 1 U/mg (1 U catalyzes the liberation of 1 µmole reducing groups from xylan per hour at 37 °C, pH 5.5, calculated as xylose).
Onozuka R-10^® is a registered trademark of Yakult Pharmaceuticals Industry Co., Ltd..


References:

1. Beldman, G. et al. (1985) Eur. J. Biochem. 146, 301-8
2. Potrykus, J. & Shillito, R.D. (1986) Methods Enzymol. 118, 549-78
3. Okada, G. (1988) Methods Enzymol. Vol. 160, 259-63
4. Lendl & Bauer (1989) Zell- und Gewebekultur, Gustav Fischer Verlag, 147ff.

DANGER

Hazard Statements	H334
Precautions	P261 - P285
Reaction	P304 +P341 - P342 +P311

EG-Index: 647-002-00-3 • EINECS: 232-734-4 • WGK: 1 • HS: 35079090
Storage Temperature: +2 °C to +8 °C