CF®568 Donkey Anti-Rat IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL

Referência 20092

Tamanho : 500uL

Marca : Biotium

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Donkey Anti-Rat IgG (H+L), Highly Cross-Adsorbed

Highly cross-adsorbed donkey anti-rat IgG (H L) secondary antibody labeled with our superior CF® Dyes.

Technology

CF® Dyes
Considerations for Immunofluorescence Staining
Secondary Antibody Product Listings

Tech Tips

Protocol: Fluorescent Western Blotting
Protocol: Immunofluorescence Staining of Cells for Microscopy
Tech Tip: Combined Direct and Indirect IF Using Primary Antibodies from the Same Host

Product Description

This is a highly cross-adsorbed donkey anti-rat IgG (H L) secondary antibody labeled with our bright and photostable CF® Dyes. To minimize cross-reactivity, the antibody has been adsorbed against bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rabbit, and sheep serum.

  • Highly cross-adsorbed for specific staining with minimal background
  • Available in 14 bright and photostable CF® Dyes
  • Suitable for western, immunofluorescence, and immunohistology in FFPE tissues

Note: Conjugates of blue fluorescent dyes like CF®350 are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Donkey Anti-Rat IgG (H+L), Highly Cross-Adsorbed

Conjugation Ex/Em Size Catalog No. Dye Features
CF®350 347/448 nm 50 uL (100 ug) 20361-50uL CF®350 Features
0.5 mL (1 mg) 20361-500uL
1 mg 20361-1mg
CF®405S 404/431 nm 50 uL (100 ug) 20419-50uL CF®405S Features
0.5 mL (1 mg) 20419-500uL
1 mg 20419-1mg
CF®488A 490/515 nm 50 uL (100 ug) 20027-1 CF®488A Features
0.5 mL (1 mg) 20027
1 mg 20027-1mg
CF®543 541/560 nm 50 uL (100 ug) 20320-1 CF®543 Features
0.5 mL (1 mg) 20320
1 mg 20320-1mg
CF®568 562/583 nm 50 uL (100 ug) 20092-1 CF®568 Features
0.5 mL (1 mg) 20092
1 mg 20092-1mg
CF®583R 585/609 nm 50 uL (100 ug) 20896-50uL CF®583R Features
0.5 mL (1 mg) 20896-500uL
CF®594 593/614 nm 50 uL (100 ug) 20159-1 CF®594 Features
0.5 mL (1 mg) 20159
1 mg 20159-1mg
CF®633 630/650 nm 50 uL (100 ug) 20137-1 CF®633 Features
0.5 mL (1 mg) 20137
1 mg 20137-1mg
CF®640R 642/662 nm 50 uL (100 ug) 20199-1 CF®640R Features
0.5 mL (1 mg) 20199
1 mg 20199-1mg
CF®647 650/665 nm 50 uL (100 ug) 20843-50uL CF®647 Features
0.5 mL (1 mg) 20843-500uL
1 mg 20843-1mg
CF®660R 663/682 nm 50 uL (100 ug) 20390-50uL CF®660R Features
0.5 mL (1 mg) 20390-500uL
1 mg 20390-1mg
CF®680 681/698 nm 50 uL (100 ug) 20417-50uL CF®680 Features
0.25 mL (500 ug) 20417-250uL
CF®740 742/767 nm 50 uL (100 ug) 20989-50uL CF®740 Features
0.25 mL (500 ug) 20989-250uL
CF®750 755/777 nm 50 uL (100 ug) 20857-50uL CF®750 Features
0.25 mL (500 ug) 20857-250uL

View our full selection of Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.

CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

Storage and Handling

Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.

Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:

CF® Dye and biotin conjugates: add 0.5 mL dH2O

HRP or DNP conjugates: add 1 mL dH2O

Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.

Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.

Note: Storage of the antibody for more than a day at final working dilution is not recommended.

CF is a registered trademark of Biotium, Inc.

References

Download a list of curated CF® Dye references.

 

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