Reagentes e instrumentos para imunologia, biologia celular e biologia molecular.

Complement C3, C3c (Complement Component C3c, C3/C3c, Acylation Stimulating Protein Cleavage Product, ASP, Complement Component 3, Complement Factor 3, CPAMD1) (Rhodamine)

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Referência C7850-14H7-1ml

Tamanho : 1ml

Contactar o distribuidor local :

Telefone : +1 850 650 7790

Marca : US Biological

Contactar o distribuidor local :

Telefone : +1 850 650 7790

C7850-14H7 Rabbit Anti-Complement C3, C3c (Complement Component C3c, C3/C3c, Acylation Stimulating Protein Cleavage Product, ASP, Complement Component 3, Complement Factor 3, CPAMD1) (Rhodamine)

Clone Type

Polyclonal

Host

goat

Source

human

Isotype

IgG

Grade

Purified

Applications

E FLISA

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

C3c is the major fragment resulting from C3 cleavage by C3 convertase and factor I. It is composed of an intact beta chain bound to two fragments of the alpha chain. ||Applications: |Suitable for use in FLISA, Immunoelectrophoresis and Double Radial Immunodiffusion. Other applications not tested.||Recommended Dilution:|Optimal dilutions to be determined by the researcher.||Excitation: |554nm ||Emission: |573nm||Molar Ratio: |~1.4||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Rhodamine conjugates are sensitive to light.

Applications

Product Type: Pab|Isotype: IgG|Host: goat|Source: human|Concentration: Not determined|Form: Supplied as a lyophilized powder from PBS, pH 7.2. Preservative free. Labeled with Rhodamine. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.|Purity: Purified|Immunogen: C3c isolated and purified from pooled normal human serum.|Specificity: Recognizes human C3c. In immunoelectrophoresis against fresh human serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single|precipitin line in the alpha-2 region is obtained. Can also react with fragments C3b, C3bi and|smaller fragments, since they all carry antigenic determinants of the C3c domain. Does not react with any other proteins component of human serum or plasma.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

C3c isolated and purified from pooled normal human serum.

Form

Supplied as a lyophilized powder from PBS, pH 7.2. Preservative free. Labeled with Rhodamine. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.

Purity

Purified

Specificity

Recognizes human C3c. In immunoelectrophoresis against fresh human serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single|precipitin line in the alpha-2 region is obtained. Can also react with fragments C3b, C3bi and|smaller fragments, since they all carry antigenic determinants of the C3c domain. Does not react with any other proteins component of human serum or plasma.