Creatininase, Microorganism (Creatinine amidohydrolase; CAH), namely creatinine amidohydrolase, from Pseudomonas putida, is a homohexameric enzyme commonly used in biochemical research. Creatininase acts on carbon-nitrogen bonds other than peptide bonds, and can catalyze the hydrolysis of creatinine to creatine, which can then be metabolized by creatinase to urea and sarcosine^[1].

Product Information
Molecular weight: 29 kDa (SDS-PAGE)
Isoelectric point: 5.3
Km value: 5.0×10^-2 M (Creatinine), 8.0×10^-2 M (Creatine)
Inhibitors: Hg²⁺, Cu²⁺, Fe³⁺
Optimum pH: 7.0-8.0
Optimum temperature: 65℃
pH stability: pH 5.5-10.0 (25℃, 16 h)
Thermal stability: stable below 65℃ (pH 8.0, 30 min)

Instructions
The enzyme can be dissolved in pure water (below 50 mg/mL concentration), or in buffer according to the specific experimental references.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

• [1]. Barbara Beuth, et al. Crystal structure of creatininase from Pseudomonas putida: a novel fold and a case of convergent evolution. J Mol Biol. 2003 Sep 5;332(1):287-301.  [Content Brief]