Dolichos biflorus (DBA) (AP)

Alkaline phosphatase (AP) is conjugated to Dolichos biflorus Lectin (DBA) to show the binding of DBA in many applications including Western blotting and ELISA. Alkaline phosphatase is a large protein (140kD) that catalyzes the hydrolysis of phosphate groups from a substrate resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein is between pH 8 and 10, and its reaction rate remains linear, improving sensitivity over time.||Source:|Horse gram||Blood Specificity:|A1 >> A2||Sugar Specificity:|alpha-N-Acetylgalactosamine||Inhibitory Carbohydrate:|N-Acetylgalactosamine||Divalent Ions:|Ca++, Mn++, Mg++, Zn++||Storage and Stability:|Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 6 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.