ExoBrite™ 410/450 CD63 Flow Antibody (25 tests)
Referência P004-410-125
Tamanho : 125UL
Marca : Biotium
Contactar o distribuidor local :
Telefone : +1 850 650 7790
| Antibody number | #P004 |
|---|---|
| SwissProt | P08962 |
| Antibody type | Primary |
| Clonality | Monoclonal |
| Host species | Mouse |
| Isotype | IgG1, kappa |
| Antibody reactivity (target) | CD63 |
| Synonyms | gp55; granulophysin; Lysosomal-associated membrane protein 3 (LAMP-3); Mast cell antigen AD1; melanoma 1 antigen; Melanoma-associated antigen MLA1; Melanoma-associated antigen ME491; MLA1; NGA; Ocular melanoma-associated antigen; OMA81H; PTLGP40; Tetraspanin-30; TSPAN30 |
| Species reactivity | Baboon, Cynomolgus monkey, Human, Non-human primates |
| Human gene symbol | CD63 |
| Entrez gene ID | 967 |
| Unigene | 445570 |
| Molecular weight | 26 kDa (core protein); 30-60 kDa (glycosylated) |
| Antibody target cellular localization | Exosomes/EVs, Lysosomes, Plasma membrane, Membrane/vesicular, Multivesicular bodies |
| Cell/tissue expression | Exosomes, Platelets, Granulocytes, Lymphocytes, Monocytes/macrophages |
| Verified antibody applications | Exosome staining (verified) |
| Shipping condition | Room temperature |
| Positive control | MCF-7 cells, MCF-7 derived exosomes |
| Antibody application notes | Recommended concentration for flow cytometry: 5 uL per 0.1 mL exosomes |
| Antibody research areas | Exosomes/EVs |
| Antibody/conjugate formulation | Proprietary buffer containing 0.05% sodium azide |
| Shelf life | Guaranteed for at least 24 months from date of receipt when stored as recommended |
| Storage Conditions | Store at 2 to 8 °C, Protect fluorescent conjugates from light |
| Regulatory status | For research use only (RUO) |
| Product origin | Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot. |
Product Description
ExoBrite™ CD63 Flow Antibody is validated by Biotium for optimal detection of EV marker CD63 in purified or bead-bound EVs by flow cytometry. The antibody conjugates offer exceptional signal-to-noise and include color options for the Pacific Blue™, FITC, and PE channels.
- NEW: Now with updated buffer formulation for improved signal-to-noise
- Optimal detection of EV marker CD63 by flow cytometry
- Validated for purified or bead-bound EVs
- Bright signal and low background
- Available in 3 colors
The most common proteins used as EV markers are CD9, CD63, and CD81, members of the tetraspanin family. These tetraspanins are broadly expressed on many cell types and can therefore be detected on many types of EVs, but their expression levels vary depending on the cell type of origin.
EV antibodies you can trust
Other commercially available antibodies for tetraspanin proteins CD9, CD63, and CD81 are generally not validated for isolated EVs and may require tedious optimization for your EV prep and staining protocol. The antibodies and dye labels of ExoBrite™ Flow Antibody Conjugates were carefully selected and validated for robust detection of isolated EVs. In addition, the antibodies are provided in a proprietary buffer formulation for reduced antibody aggregation and brighter EV staining for optimal accuracy and signal-to-noise.
ExoBrite™ Isotype Control Flow Antibodies
, which have been found to not react with any target in human cells and have the same isotype as the tetraspanin antibodies, are also available as a negative control.For general EV staining, Biotium’s
ExoBrite™ True EV Membrane Stains
offer unparalleled coverage of EVs in a sample and address issues of dye aggregation often seen with PKH and other common membrane dyes. Biotium also offers optimized ExoBrite™ EV Surface stains conjugated tocholera toxin B (CTB)
,wheat germ agglutinin (WGA)
, andAnnexin V
. These stains are specially formulated for bright and specific detection of isolated EVs by flow cytometry. These ExoBrite™ EV Surface stains may also be combined with antibody staining, for multi-parameter analysis.Biotium also offers conjugated ExoBrite™ Western Antibodies against
CD9
,CD63
, andCD81
designed for optimal detection in EV extracts by fluorescent western blot.Note: In our testing, we have found that ExoBrite™ 490/515 dye may bind to streptavidin coated surfaces or beads if free biotin binding sites are not blocked. We recommend performing a biotin blocking step after binding your biotinylated capture antibody to streptavidin beads or surfaces when using ExoBrite™ 490/515 conjugates. Alternatively, consider using a different ExoBrite™ dye for staining EVs captured on streptavidin beads or surfaces.
ExoBrite™ Flow Antibody Conjugates
| Antibody | Ex/Em | Detection Channel | Size | Catalog No. |
|---|---|---|---|---|
ExoBrite™ 410/450 | 416/452 nm | Pacific Blue ™ | 25 tests | P003-410-125 |
| 100 tests | P003-410-500 | |||
ExoBrite™ 490/515 | 490/516 nm | FITC | 25 tests | P003-490-125 |
| 100 tests | P003-490-500 | |||
ExoBrite™ 560/585 | 562/584 nm | PE | 25 tests | P003-560-125 |
| 100 tests | P003-560-500 | |||
ExoBrite™ 650/665 | 652/668 nm | APC | 25 tests | P003-650-125 |
| 100 tests | P003-650-500 | |||
ExoBrite™ R-PE | 496, 546, 565/578 | PE | 25 tests | P003-RPE-125 |
| 100 tests | P003-RPE-500 | |||
ExoBrite™ 410/450 | 416/452 nm | Pacific Blue ™ | 25 tests | P018-410-125 |
| 100 tests | P018-410-500 | |||
ExoBrite™ 490/515 | 490/516 nm | FITC | 25 tests | P018-490-125 |
| 100 tests | P018-490-500 | |||
ExoBrite™ 560/585 | 562/584 nm | PE | 25 tests | P018-560-125 |
| 100 tests | P018-560-500 | |||
ExoBrite™ 650/665 | 652/668 nm | APC | 25 tests | P018-650-125 |
| 100 tests | P018-650-500 | |||
ExoBrite™ 410/450 | 416/452 nm | Pacific Blue ™ | 25 tests | P004-410-125 |
| 100 tests | P004-410-500 | |||
ExoBrite™ 490/515 | 490/516 nm | FITC | 25 tests | P004-490-125 |
| 100 tests | P004-490-500 | |||
ExoBrite™ 560/585 | 562/584 nm | PE | 25 tests | P004-560-125 |
| 100 tests | P004-560-500 | |||
ExoBrite™ R-PE | 496, 546, 565/578 | PE | 25 tests | P004-RPE-125 |
| 100 tests | P004-RPE-500 | |||
ExoBrite™ 410/450 | 416/452 nm | Pacific Blue ™ | 25 tests | P022-410-125 |
| 100 tests | P022-410-500 | |||
ExoBrite™ 490/515 | 490/516 nm | FITC | 25 tests | P022-490-125 |
| 100 tests | P022-490-500 | |||
ExoBrite™ 560/585 | 562/584 nm | PE | 25 tests | P022-560-125 |
| 100 tests | P022-560-500 | |||
ExoBrite™ 410/450 | 416/452 nm | Pacific Blue ™ | 25 tests | P005-410-125 |
| 100 tests | P005-410-500 | |||
ExoBrite™ 490/515 | 490/516 nm | FITC | 25 tests | P005-490-125 |
| 100 tests | P005-490-500 | |||
ExoBrite™ 560/585 | 562/584 nm | PE | 25 tests | P005-560-125 |
| 100 tests | P005-560-500 | |||
ExoBrite™ R-PE | 496, 546, 565/578 | PE | 25 tests | P005-RPE-125 |
| 100 tests | P005-RPE-500 | |||
ExoBrite™ 410/450 | 416/452 nm | Pacific Blue ™ | 25 tests | P019-410-125 |
| 100 tests | P019-410-500 | |||
ExoBrite™ 490/515 | 490/516 nm | FITC | 25 tests | P019-490-125 |
| 100 tests | P019-490-500 | |||
ExoBrite™ 560/585 | 562/584 nm | PE | 25 tests | P019-560-125 |
| 100 tests | P019-560-500 | |||
ExoBrite™ 410/450 IgG1 | 416/452 nm | Pacific Blue ™ | 25 tests | P008-410-125 |
| 100 tests | P008-410-500 | |||
ExoBrite™ 490/515 IgG1 | 490/516 nm | FITC | 25 tests | P008-490-125 |
| 100 tests | P008-490-500 | |||
ExoBrite™ 560/585 IgG1 | 562/584 nm | PE | 25 tests | P008-560-125 |
| 100 tests | P008-560-500 | |||
ExoBrite™ R-PE IgG1 | 496, 546, 565/578 | PE | 25 tests | P008-RPE-125 |
| 100 tests | P008-RPE-500 | |||
ExoBrite™ 650/665 IgG1 | 652/668 nm | APC | 25 tests | P008-650-125 |
| 100 tests | P008-650-500 |