FGF1 Chemi-Luminescent ELISA Kit (Chicken)
Referência OKCD03458
Tamanho : 96Wells
Marca : Aviva Systems Biology
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Telefone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for FGF1 Chemi-Luminescent ELISA Kit (Chicken) (OKCD03458) |
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| Predicted Species Reactivity | Chicken|Gallus gallus | ||||||||||||||||||||||
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| Application | CLIA | ||||||||||||||||||||||
| ELISA Kit Detection Method | Chemiluminescent | ||||||||||||||||||||||
| ELISA Kit Duration | 2h, 40min | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | The microplate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 1, Acidic (FGF1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 1, Acidic (FGF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fibroblast Growth Factor 1, Acidic (FGF1) level in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 4.12-3,000pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 1, Acidic (FGF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 1, Acidic (FGF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 46.88 pg/mL | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | FGF1 |
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| Gene Full Name | fibroblast growth factor 1 |
| Alias Symbols | Acidic fibroblast growth factor;acidic fibroblast growth factor, aFGF;aFGF;alpha-endothelial cell growth factor;ECGF;endothelial cell growth factor;FGF-1;fibroblast growth factor 1;fibroblast growth factor 1 (acidic);HBGF-1;heparin-binding growth factor 1. |
| NCBI Gene Id | 396094 |
| Protein Name | Fibroblast growth factor 1 |
| Description of Target | Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrins. Its binding to integrins and subsequent ternary complex formation with integrins and FGFR1 are essential for FGF1 signaling. |
| Uniprot ID | P19596 |
| Protein Accession # | NP_990511 |
| Nucleotide Accession # | NM_205180 |