Hormone Abscisic Acid ELISA Kit (Plant)

Referência OKCA00373

Tamanho : 96Wells

Marca : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetClickHereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityPlant
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration< 4 Hours
ELISA Kit PrincipleAviva Systems Biology Hormone Abscisic Acid ELISA Kit (Plant) (OKCA00373) is based on a competitive enzyme immuno assay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for Hormone Abscisic Acid and Horseradish Peroxidase (HRP) conjugated Hormone Abscisic Acid. The competitive inhibition reaction is launched between with HRP labeled Hormone Abscisic Acid and unlabeled Hormone Abscisic Acid with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Hormone Abscisic Acid in the sample. The color development is stopped and the intensity of the color is measured.
ELISA Kit Range0.156 ug/mL - 10 ug/mL
ELISA Kit Recovery
Sample 1
Sample TypeAverage RecoveryRange
Xylem saps from plants (n=5)0.9589-98%
Sample 2
Sample TypeAverage RecoveryRange
Crude extracts (n=5)0.9590-100%
ELISA Kit Reproducibility

Mean Intra-Assay Precision %CV: < 10% (n = 20)

Mean Inter-Assay Precision %CV: < 20% (n = 20)

ELISA Kit Component
ComponentAmount
Anti-Hormone Abscisic Acid Microplate96 Well (12 x 8 Well Strips)
10X Hormone Abscisic Acid Standard1 x 200 uL
100X Antibody 1 x 60 uL
100X Anti-Hormone Abscisic Acid Antibody HRP Conjugate1 x 120 uL
Antibody Diluent1 x 10 mL
25X HRP-Conjugate Diluent1 x 20 mL
Sample Extraction Buffer 1 x 20 mL
Sample Diluent2 x 20 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional Information

CAS#: 21293-29-8

Molecular Formula: C15H20O4

Molecular Weight: 264.32 g·mol−1

Reconstitution and StorageStore as indicated in product manual.
Sample TypePlant tissues
Sensitivity< 0.04 ug/mL
Assay InfoAssay Methodology: Quantitative Competitive Immunoassay
Gene SymbolHormone Abscisic Acid
Alias SymbolsAbscisic Acid, Abscisic Acid Monoammonium Salt, (R)-Isomer, Abscisic Acid, (+,-)-Isomer, Abscisic Acid, (E,E)-(+-)-Isomer, Abscisic Acid, (E,Z)-(+,-)-Isomer, Abscisic Acid, (R)-Isomer, Abscisic Acid, (Z,E)-Isomer, Abscissic Acid, Abscissins
Protein NameHormone Abscisic Acid
Description of TargetAbscission-accelerating plant growth substance isolated from young cotton fruit, leaves of sycamore, birch, and other plants, and from potatoes, lemons, avocados, and other fruits.

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