PC2002-25ml | HyperChrom Ni-NTA HP Agarose Clinisciences

Description

HyperChrom Ni-NTA HP Agarose is an affinity chromatography medium made by chemically coupling the transition metal ion Ni2+ with tetracoordination to a ligand of Nitrilotriacetic acid (NTA) using a highly crosslinked agarose gel as a matrix. It has a very stable octahedral structure, nickel ions are at the center of the octahedron, which protects nickel ions from attack by small molecules, is more stable, and can withstand harsh conditions such as reducing agents, denaturants or couplants in a certain concentration. In addition, due to the pressure resistance of the matrix, which can withstand pressures up to 0.3 MPa, the product can be used for industrial large-scale protein purification, enabling purification of the protein of interest at relatively high flow rates. This chromatography medium uses the interaction between Ni2+ and the side chains of certain amino acids (mainly histidine) on the protein to achieve the separation and purification of proteins containing and not containing these amino acids and containing a large and low number of these amino acids.

HyperChrom Ni-NTA HP Agarose is the first choice for purifying histidine-tagged proteins, with the advantages of high load, easy regeneration, and low cost. Compared to HyperChrom Ni-NTA FF Agarose, HyperChrom Ni-NTA HP Agarose has a finer particle size and provides higher resolution.

HyperChrom Ni-NTA HP Agarose chromatography media parameters

Name	Description
Chromatography media type	Affinity chromatography media
Ligation	NTA-Ni²⁺
Scaffolding	Highly cross-linked agarose
Average particle size	34 μm
Ligand density	~15 μmol Ni²⁺/mL chromatography medium
Dynamic load	≥40 mg histidine-tagged protein/mL chromatography medium*
Flow rates are recommended	90-150 cm/h
Maximum flow rate	200 cm/h
Withstand pressure	0.3 MPa
Use temperature	4 - 30℃
pH stability^**	2-14
Chemical resistance	Common aqueous solution, 0.01 M HCl, 0.1 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol ***
* Dynamic load measurement conditions: column loading height: 10 cm, test flow rate 150 cm/h; Test buffer: 0.02 M PB, 0.5 M NaCl, 5 mM imidazole, pH 7.4; test sample: tagged protein (1 mg/ml, 6His); Index: When the protein penetration reaches 10%, the amount of protein loaded per unit volume of media (mL) (mg). After 7 days in the environment of chromatography at 40°C and pH 2-14, the physical and chemical properties and functions of the chromatography medium did not change significantly. ** 30% is v/v, volume ratio.

Quality Control

Protocol

Components and Storage

Components	PC2002-25 mL	PC2002-100 mL
HyperChrom Ni-NTA HP Agarose	25 mL	100 mL
Store the components at 4℃ for 5 years.

HyperChrom Ni-NTA HP Agarose

Referência PC2002-25ml

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Description

Quality Control

Components and Storage