IFNA1 ELISA Kit (Human)

Referência OKEH00052

Tamanho : 96T

Marca : Aviva Systems Biology

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Telefone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IFNA1 ELISA Kit (Human) (OKEH00052) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IFNA1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IFNA1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IFNA1 captured in well.
ELISA Kit Range7.8-500pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.9% (n = 20)
Mean Inter-assay CV%: < 6.6% (n = 20)
ELISA Kit Component
ComponentAmount
IFNA1 Microplate96 Wells (12 x 8 Well strips)
IFNA1 Lyophilized Standard2
100X Biotinylated IFNA1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity1.95 pg/mL
SpecificityNatural and recombinant Human Interferon alpha-1/13
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIFNA1, IFNA13
Gene Full Nameinterferon alpha 1, interferon alpha 13
Alias SymbolsIFL;IFN;IFNA@;IFNA13;IFN-ALPHA;IFN-alpha-1/13;IFN-alphaD;interferon alpha 1b;interferon alpha-1/13;interferon alpha-D;interferon-alpha1;leIF D.
NCBI Gene Id3439, 3447
Protein NameP01562
Description of TargetInterferon alpha-1/13
Uniprot IDhttps://www.uniprot.org/uniprot/P01562
Protein Accession #NP_076918
Nucleotide Accession #NM_024013
Protein Size (# AA)189
Molecular Weight22 kDa

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Price Bef. VAT
OKEH00140
 96Wells