IL2 Receptor alpha antibody [PC61.5] (PerCP-Cy5.5)
Referência GTX01472-11-100ug
Tamanho : 100ug
Marca : GeneTex
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Telefone : +1 850 650 7790
![Anti-IL2 Receptor alpha antibody [PC61.5] (PerCP-Cy5.5) used in Flow cytometry (FACS). GTX01472-11](/proxy_img/aHR0cDovL3d3dy5nZW5ldGV4LmNvbS91cGxvYWQvd2Vic2l0ZS9wcm91Y3RfaW1nL3NtYWxsL0dUWDAxNDcyLTExL0dUWDAxNDcyLTExXzIwMjAwNDI4X0ZBQ1MxNTlfd18yMzA1MzEyMV82NjIud2VicA==.webp "Anti-IL2 Receptor alpha antibody [PC61.5] (PerCP-Cy5.5) used in Flow cytometry (FACS). GTX01472-11")
Host | Rat |
|---|---|
Clonality | Monoclonal |
Clone Name | PC61.5 |
Isotype | IgG1 |
Application | FACS |
Reactivity | Mouse |
| Package | 100 μg |
Datasheet File View product citations for antibody GTX01472-11 on CiteAb
PRODUCT
Summary
The PC61.5 antibody is specific for mouse CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, although with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Expression of CD25 varies during developmental stages of T and B cells, is induced on activated mature T and B cells, and is present on subsets of dendritic cells. CD25 signaling as part of the IL-2 receptor complex triggers T cell activation and proliferation, as well as modulating the differentiation and function of Th17 cells, T regulatory (Treg) cells, and dendritic cells. The PC61.5 antibody is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used to distinguish Treg cells from naïve or conventional T cells which are CD25. This clone has also been reported for depletion of Treg cells in vivo.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application | Recommended Dilution |
|---|---|
| FACS | Assay dependent |
Not tested in other applications.
PROPERTIES
Form
Liquid
Buffer
10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Purification
Purified by affinity chromatography
From tissue culture supernatant
From tissue culture supernatant
Conjugation
Peridinin-chlorophyll proteins-Cyanine5.5 (PerCP-Cy5.5) Wavelength
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Cellular Localization
Membrane
Database
DATA IMAGES
![Anti-IL2 Receptor alpha antibody [PC61.5] (PerCP-Cy5.5) used in Flow cytometry (FACS). GTX01472-11](/proxy_img/aHR0cDovL3d3dy5nZW5ldGV4LmNvbS91cGxvYWQvd2Vic2l0ZS9wcm91Y3RfaW1nL21pZGRsZS9HVFgwMTQ3Mi0xMS9HVFgwMTQ3Mi0xMV8yMDIwMDQyOF9GQUNTMTU5X3dfMjMwNTMxMjFfNjYyLndlYnA=.webp "Anti-IL2 Receptor alpha antibody [PC61.5] (PerCP-Cy5.5) used in Flow cytometry (FACS). GTX01472-11") | GTX01472-11 FACS Image FACS analysis of mouse C57Bl/6 splenocytes using GTX01472-11 IL2 Receptor alpha antibody [PC61.5] (PerCP-Cy5.5). |
Clone Reference
2012; Anti-IL-7 receptor-α reverses established type 1 diabetes in nonobese diabetic mice by modulating effector T-cell function.
2012; Increased CD8+ T-cell function following castration and immunization is countered by parallel expansion of regulatory T cells.
2012; IL-21 inhibits T cell IL-2 production and impairs Treg homeostasis.
2012; Simultaneous inhibition of two regulatory T-cell subsets enhanced Interleukin-15 efficacy in a prostate tumor model.
2012; Role of CD25+ dendritic cells in the generation of Th17 autoreactive T cells in autoimmune experimental uveitis.
Submit a Reference REVIEW
Clone Reference
2012; Anti-IL-7 receptor-α reverses established type 1 diabetes in nonobese diabetic mice by modulating effector T-cell function.
2012; Increased CD8+ T-cell function following castration and immunization is countered by parallel expansion of regulatory T cells.
2012; IL-21 inhibits T cell IL-2 production and impairs Treg homeostasis.
2012; Simultaneous inhibition of two regulatory T-cell subsets enhanced Interleukin-15 efficacy in a prostate tumor model.
2012; Role of CD25+ dendritic cells in the generation of Th17 autoreactive T cells in autoimmune experimental uveitis.
2008; PD-1-dependent mechanisms maintain peripheral tolerance of donor-reactive CD8+ T cells to transplanted tissue.
2006; Foxp3-expressing CD103+ regulatory T cells accumulate in dendritic cell aggregates of the colonic mucosa in murine transfer colitis.
1986; Dissociation of interleukin 2-dependent and -independent B cell proliferation with monoclonal anti-interleukin 2 receptor antibody.
1985; Similarities between interleukin-2 receptor number and affinity on activated B and T lymphocytes.
1985; Expression of interleukin-2 receptors as a differentiation marker on intrathymic stem cells.
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| Sodium Azide.pdf | |